Modified Mayer's Hematoxylin
(HB6189)
Product overview
| Name | Modified Mayer's Hematoxylin |
| Alternative names | Hematoxylin Solution (Mayer's, Modified) |
| Biological description | Hematoxylin is a nuclear stain with a deep blue-purple color. It is used as a counterstain for immunohistochemical or hybridization procedures that use colorimetric substrates (e.g. alkaline phosphatase or peroxidase.) It is a component of one of the most widely used histology stains: the H&E stain (Hematoxylin and Eosin stain). |
| Description | Nuclear stain. Component of H&E stain. |
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Images
Figure 1. Hematoxylin staining in mouse striatum
Modified Mayer's Hematoxylin nuclear staining in paraffin embedded mouse brain tissue. Modified Mayer's Hematoxylin from Hello Bio stains nuclei blue-purple with crisp detail.
Figure 2. Parvalbumin positive neurons visualized in rat cerebellum using DAB staining using hematoxylin counterstain
Figure 3. Modified Mayer's Hematoxylin (HB6189) nuclear staining in mesenteric connective tissue.
Modified Mayer's Hematoxylin nuclear staining in mesenteric connective tissue. Modified Mayer's Hematoxylin from Hello Bio stains nuclei blue-purple with crisp detail.
Figure 4. Tyrosine hydroxylase staining in mouse striatum with hematoxylin counterstain
Paraffin embedded mouse brain section stained for tyrosine hydroxylase using HB6605 showing the dense network of dopaminergic neurons in the mouse striatum. DAB (HB0687) was used to develop the reaction and Modified Mayer's Hematoxylin was used as a nuclear counterstain.
Figure 5. Parvalbumin positive neurons visualized in rat cerebellum using DAB staining using hematoxylin counterstain
Figure 6. Tyrosine hydroxylase staining in mouse striatum with hematoxylin counterstain
Paraffin embedded mouse brain section stained for tyrosine hydroxylase using HB6605 showing the dense network of dopaminergic neurons in the mouse striatum. DAB (HB0687) was used to develop the reaction and Modified Mayer's Hematoxylin was used as a nuclear counterstain.
Figure 1. Hematoxylin staining in mouse striatum
Modified Mayer's Hematoxylin nuclear staining in paraffin embedded mouse brain tissue. Modified Mayer's Hematoxylin from Hello Bio stains nuclei blue-purple with crisp detail.
Figure 2. Parvalbumin positive neurons visualized in rat cerebellum using DAB staining using hematoxylin counterstain
Figure 3. Modified Mayer's Hematoxylin (HB6189) nuclear staining in mesenteric connective tissue.
Modified Mayer's Hematoxylin nuclear staining in mesenteric connective tissue. Modified Mayer's Hematoxylin from Hello Bio stains nuclei blue-purple with crisp detail.
Figure 4. Tyrosine hydroxylase staining in mouse striatum with hematoxylin counterstain
Paraffin embedded mouse brain section stained for tyrosine hydroxylase using HB6605 showing the dense network of dopaminergic neurons in the mouse striatum. DAB (HB0687) was used to develop the reaction and Modified Mayer's Hematoxylin was used as a nuclear counterstain.
Figure 5. Parvalbumin positive neurons visualized in rat cerebellum using DAB staining using hematoxylin counterstain
Figure 6. Tyrosine hydroxylase staining in mouse striatum with hematoxylin counterstain
Paraffin embedded mouse brain section stained for tyrosine hydroxylase using HB6605 showing the dense network of dopaminergic neurons in the mouse striatum. DAB (HB0687) was used to develop the reaction and Modified Mayer's Hematoxylin was used as a nuclear counterstain.
Biological Data
| Application notes | Hematoxylin staining protocol for paraffin embedded sections Â
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Solubility & Handling
| Storage instructions | Room temperature |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Technical guides
Understanding purity and quality - a guide for life scientistsReferences for Modified Mayer's Hematoxylin
References are publications that support the biological activity of the product
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Tissue Processing and Hematoxylin and Eosin Staining
Feldman and Wolfe (2014) Methods Mol Biol 1180 : 31-43 -
The Wonderful Colors of the Hematoxylin-Eosin Stain in Diagnostic Surgical Pathology
CHan (2014) Int J Surg Pathol 22(1) : 12-32 -
Hematoxylin and Eosin Staining of Tissue and Cell Sections
Fischer et al (2008) CSH Protoc : 4986
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