Novel, caged, fast-equilibrating version of the low-affinity competitive glutamate antagonist γ-DGG. Photo-release (e.g. by flashlamp or laser photolysis) releases γ-DGG. Photo-release of MNI-caged-γDGG (at concentrations up to 5mM) releases γ-DGG at concentrations up to 1.5mM in 1ms in wide-field flashlamp photolysis at climbing fiber-Purkinje cell (CF-PC) synapses without affecting (CF-PC) transmission. Photo-released γ-DGG has been shown to inhibit the CF- first and second paired EPSCs by ~30-60% respectively at concentrations of 0.55-1.7mM.
MNI-caged γ-DGG may be used as a synaptic probe as photolysis of MNI-caged γ-DGG resolves timing and extent of transmitter activation of receptors in glutamatergic transmission.
Solubility & Handling
Solubility overview
Soluble in water
Storage instructions
-20°C
Storage of solutions
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
References for MNI-caged γ-DGG (MNI-Caged γ-D-Glutamyl-Glycine)
References are publications that support the biological activity of the product
Photolysis of a Caged, Fast-Equilibrating Glutamate Receptor Antagonist, MNI-Caged γ-D-Glutamyl-Glycine, to Investigate Transmitter Dynamics and Receptor Properties at Glutamatergic Synapses.
Palma-Cerda F et al (2018) Frontiers in cellular neuroscience 12 : 465
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