JC-10 is a highly soluble fluorescent probe ideal for assessing mitochondrial membrane potential. Â In healthy cells with polarized mitochondria, JC-10 aggregates, emitting a strong orange fluorescence (Ex/Em: 540nm/590nm). Â However, in cells with depolarized mitochondria, a hallmark of apoptosis and other cellular stresses, JC-10 reverts to its monomeric form, resulting in a shift to green fluorescence (Ex/Em: 490nm/525nm). This reversible, ratiometric change in fluorescence emission provides a reliable indicator of mitochondrial health. Â JC-10's superior aqueous solubility to JC-1 makes it a convenient and robust tool for various applications, including fluorescence microscopy, flow cytometry, and high-throughput screening.
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This kit contains everything needed to make 25 mL of working solution which is suitable for five 96-well plates or 500 flow cytometry samples.
Figure 1. Effects of staurosporine upon HeLa mitochondrial membrane potential
Staurosporine is an apoptosis inducing protease kinase inhibitor which leads to an increase in mitochondrial membrane depolarization. Cells were measured both after 8 hours and 24 hours with increased membrane depolarization after 24 hours. For more information please see our JC-10 Mitochondrial Membrane Potential Assay Kit Protocol
Figure 2. Effects of CCCP (carbonyl cyanide m-chlorophenyl hydrazone) upon the mitochondrial membrane potential of treated cells measured with JC-10.
CCCP (HB5062HB5062, carbonyl cyanide m-chlorophenyl hydrazone) is a potent mitochondrial decoupling agent which works by forming a proton pore across the mitochondrial inner membrane leading to mitochondrial depolarisation. Incubation with CCCP for 4 hours leads to a decreased mitochondrial membrane potential as measured by an increase in orange/green fluorescence with JC-10. For more information please see our JC-10 Mitochondrial Membrane Potential Assay Kit Protocol
Figure 1. Effects of staurosporine upon HeLa mitochondrial membrane potential
Staurosporine is an apoptosis inducing protease kinase inhibitor which leads to an increase in mitochondrial membrane depolarization. Cells were measured both after 8 hours and 24 hours with increased membrane depolarization after 24 hours. For more information please see our JC-10 Mitochondrial Membrane Potential Assay Kit Protocol
Figure 2. Effects of CCCP (carbonyl cyanide m-chlorophenyl hydrazone) upon the mitochondrial membrane potential of treated cells measured with JC-10.
CCCP (HB5062HB5062, carbonyl cyanide m-chlorophenyl hydrazone) is a potent mitochondrial decoupling agent which works by forming a proton pore across the mitochondrial inner membrane leading to mitochondrial depolarisation. Incubation with CCCP for 4 hours leads to a decreased mitochondrial membrane potential as measured by an increase in orange/green fluorescence with JC-10. For more information please see our JC-10 Mitochondrial Membrane Potential Assay Kit Protocol
JC-10 is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
