Asante Natrium Green, Ion Natrium Green, ANG, ING, ANG-1, ING-1
Biological description
Membrane permeable, yellow-green fluorescent (Excitation 525nm, Emission 545nm), intracellular sodium (Na⁺) indicator (Kd = 20mM). Has improved cellular loading and significantly higher brightness than SBFI. Suitable for high-throughput screening applications targeting Na⁺ channels, and non-selective monovalent cation channels due to its spectral properties and large dynamic range. Also compatible with fluorescence microscopy using common fluorescein, GFP or more ideally YFP filters.
Figure 1. Intracellular Na+ accumulation caused by Na+/K+ ATPase inhibition in CHO K1 cells measured with ING-2
Oubain is a potent Na+/K+ ATPase inhibitor which increases intracellular Na+ concentrations and therefore ING-2 fluorescence at 545nm in a dose-dependent manner. Following a baseline period, oubain was added and fluorescence measured for 4.5 minutes. Data is shown as mean ± standard deviation. For more details please see our ING-2 protocol.
Figure 2. Concentration response of ING-2 fluorescence in response to changing Na+ concentration.
ING-2 fluorescence at 545nm was measured in response to changing Na+ concentration in 12.5mM Tris buffer pH7.4 containing 0.25% BSA, 1.2mM Mg2+ and TMA+ to maintain a constant ion concentration of 150mM. This resulted in a Kd of 5mM.
Figure 3. ING-2 response to monovalent and divalent metal cations.
ING-2 was screened against a panel of monovalent and divalent metal cations for increased fluorescence with ion concentration. Measurements were made in 140mM TMACI, 10mM Tris-HCl, 3µM ING-2 for monovalent cations and 10mM EGTA, 10mM MOPS, 100mM K+, 3µM ING-2, pH7.2 for divalent cations. Values are expressed relative to an ion-free reference solution.
Figure 1. Intracellular Na+ accumulation caused by Na+/K+ ATPase inhibition in CHO K1 cells measured with ING-2
Oubain is a potent Na+/K+ ATPase inhibitor which increases intracellular Na+ concentrations and therefore ING-2 fluorescence at 545nm in a dose-dependent manner. Following a baseline period, oubain was added and fluorescence measured for 4.5 minutes. Data is shown as mean ± standard deviation. For more details please see our ING-2 protocol.
Figure 2. Concentration response of ING-2 fluorescence in response to changing Na+ concentration.
ING-2 fluorescence at 545nm was measured in response to changing Na+ concentration in 12.5mM Tris buffer pH7.4 containing 0.25% BSA, 1.2mM Mg2+ and TMA+ to maintain a constant ion concentration of 150mM. This resulted in a Kd of 5mM.
Figure 3. ING-2 response to monovalent and divalent metal cations.
ING-2 was screened against a panel of monovalent and divalent metal cations for increased fluorescence with ion concentration. Measurements were made in 140mM TMACI, 10mM Tris-HCl, 3µM ING-2 for monovalent cations and 10mM EGTA, 10mM MOPS, 100mM K+, 3µM ING-2, pH7.2 for divalent cations. Values are expressed relative to an ion-free reference solution.
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
ING-2 AM Protocol Book (220.7 KB) 
