Asante Potassium Green, Ion Potassium Green, APG, IPG, APG-4, IPG-4
Biological description
Membrane permeable potassium indicator (Excitation 525nm, Emission 545nm) which is compatible with a wide variety of detectors (e.g. fluorescent microscopes, plate readers, flow cytometers, and fluorescent indicator-doped solid-state sensors). Can be used with common filter sets (e.g. YFP and FITC) and multiphoton approaches. Higher affinity for potassium (Kd = 7mM) compared to IPG-2 (Kd = 18mM) Suitable for diverse applications such as extracellular K+ sensing and monitoring intracellular K+ dynamics. Synthetic fluorochrome which incorporates a K+-binding moiety. Under conditions where K+ is not bound, the fluorescence of the sensor is significantly quenched. When K+ is bound, the quenching is relieved, and the fluorescence of the sensor dramatically increases. Additionally IPG-4 is not a MDR1 (pgp) substrate therefore is compatible with probenecid free assays.
Purity
>95%
Description
Yellow-green fluorescent potassium indicator. Membrane permeable. Higher affinity than IPG-2.
Figure 1. Intracellular K+ depletion caused by GIRK channel activation in CHO cells measured with IPG-4
VU551 is a potent activator of GIRK channels expressed in CHO cells. VU551 results in a concentration dependent depletion of intracellular K+ which correlates with decreased fluorescence at 545nm from IPG-4 in a dose dependent manner. Equivalent results are gained when using either a plate reader or fluorescent microscope to collect date. For more details please see our IPG-4 protocol.
Figure 2. Concentration response of IPG-4 fluorescence in response to changing K+ concentration.
IPG-4 fluorescence at 545nm was measured in response to changing K+ concentration in 12.5mM Tris buffer pH7.4 containing 0.25% BSA, 1.2mM Mg2+ and TMA+ to maintain a constant ion concentration of 150mM. This resulted in a Kd of 4mM.
Figure 3. IPG-4 response to monovalent and divalent metal cations.
IPG-4 was screened against a panel of monovalent and divalent metal cations for increased fluorescence with ion concentration. Measurements were made in 140mM TMACI, 10mM Tris-HCl, 3µM IPG-4 for monovalent cations and 10mM EGTA, 10mM MOPS, 100mM K+, 3µM IPG-4, pH7.2 for divalent cations. Values are expressed relative to an ion-free reference solution.
Figure 1. Intracellular K+ depletion caused by GIRK channel activation in CHO cells measured with IPG-4
VU551 is a potent activator of GIRK channels expressed in CHO cells. VU551 results in a concentration dependent depletion of intracellular K+ which correlates with decreased fluorescence at 545nm from IPG-4 in a dose dependent manner. Equivalent results are gained when using either a plate reader or fluorescent microscope to collect date. For more details please see our IPG-4 protocol.
Figure 2. Concentration response of IPG-4 fluorescence in response to changing K+ concentration.
IPG-4 fluorescence at 545nm was measured in response to changing K+ concentration in 12.5mM Tris buffer pH7.4 containing 0.25% BSA, 1.2mM Mg2+ and TMA+ to maintain a constant ion concentration of 150mM. This resulted in a Kd of 4mM.
Figure 3. IPG-4 response to monovalent and divalent metal cations.
IPG-4 was screened against a panel of monovalent and divalent metal cations for increased fluorescence with ion concentration. Measurements were made in 140mM TMACI, 10mM Tris-HCl, 3µM IPG-4 for monovalent cations and 10mM EGTA, 10mM MOPS, 100mM K+, 3µM IPG-4, pH7.2 for divalent cations. Values are expressed relative to an ion-free reference solution.
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
