Red fluorescent calcium (Ca2+) indicator for intracellular Ca2+ measurements (Kd = 480nM). Has long-wavelength emission and a large Stokes shift (Excitation 580nm, Emission 660nm) which reduces contributions of autofluorescence. ICR-1 AM is optimal for cellular and tissue imaging applications and can be multiplexed with GFP-labeled cells or other green fluorophores. Does not accumulate in the mitochondria. Compatible with both fluorescence lifetime imaging and multiphoton imaging.
Purity
>95%
Description
Red fluorescent membrane permeable calcium indicator
Figure 1. P2X7 mediated increased intracellular Ca2+ with increased ATP concentration measured using ICR-1 in CHO K1 cells.
ATP activates P2X7 receptors in a dose dependent manner in CHO-K1 cells leading to increased intracellular calcium which is detected as an increase in ICR-1 fluorescence at 660nm. Cells were first pre-incubated with ICR-1 followed by a 30 second baseline recording. ATP was then added and the increase in fluorescence was measured at 660nm for a further 60 seconds. All recordings were made at 37°C. For more details please see our ICR-1 protocol.
Figure 2. Excitation and emission spectra for ICR-1
Excitation and emission spectra were recorded for ICR-1 in sodium bicarbonate buffer.
Figure 1. P2X7 mediated increased intracellular Ca2+ with increased ATP concentration measured using ICR-1 in CHO K1 cells.
ATP activates P2X7 receptors in a dose dependent manner in CHO-K1 cells leading to increased intracellular calcium which is detected as an increase in ICR-1 fluorescence at 660nm. Cells were first pre-incubated with ICR-1 followed by a 30 second baseline recording. ATP was then added and the increase in fluorescence was measured at 660nm for a further 60 seconds. All recordings were made at 37°C. For more details please see our ICR-1 protocol.
Figure 2. Excitation and emission spectra for ICR-1
Excitation and emission spectra were recorded for ICR-1 in sodium bicarbonate buffer.
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
ICR-1 AM Protocol Book (221.2 KB) 
