Product overview
| Name | Hoechst 33342 Staining Solution (10mg/ml) |
| Alternative names | H33342, Bisbenzimide H 33342 |
| Biological description | 10mg/ml staining solution. Blue fluorescent DNA stain that is commonly used in fluorescent microscopy and frequently used to stain nuclei and is cell permeable. Can be used on both live and fixed cells. Solid powder form in 50mg and 100mg also available. |
| Description | Blue fluorescent DNA stain. Nuclear stain. 10mg/ml staining solution in water. Solid also available in 50mg and 100mg. |
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Hoechst 33342 staining in mouse hippocampus
Hoechst 33342 is a DNA binding dye commonly used to label cell nuclei in immunofluorescence experiments. Hoechst 33342 from Hello Bio labels cell nuclei at 1µg/ml. For protocol see #Protocol 1 in application notes below.
Hoechst 33342 staining in mouse hippocampus
Hoechst 33342 is a DNA binding dye commonly used to label cell nuclei in immunofluorescence experiments. Hoechst 33342 from Hello Bio labels cell nuclei at 1µg/ml. For protocol see #Protocol 1 in application notes below.
Biological Data
| Application notes | #Protocol 1: Hoechst 33342 staining of mouse brain sections.
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Solubility & Handling
| Storage instructions | -20°C |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Chemical Data
| Chemical name | 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole |
| Molecular Weight | 561.93 |
| Chemical structure |  |
| Molecular Formula | C27H28N6O.3HCl |
| CAS Number | 875756-97-1 |
Technical guides
Understanding purity and quality - a guide for life scientistsReferences for Hoechst 33342 Staining Solution (10mg/ml)
References are publications that support the biological activity of the product
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Analyzing Cell Death by Nuclear Staining with Hoechst 33342.
Crowley LC et al (2016) Cold Spring Harbor protocols 2016 : -
Phototoxicity of Hoechst 33342 in time-lapse fluorescence microscopy.
Purschke M et al (2010) Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology 9 : 1634-9 -
Hoechst 33342: the dye that enabled differentiation of living X-and Y-chromosome bearing mammalian sperm.
Garner DL (2009) Theriogenology 71 : 11-21
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