Hoechst 33258 Staining Solution (10mg/ml)

(HB8505)
Technical documents: Datasheet

Product overview

Name Hoechst 33258 Staining Solution (10mg/ml)
Alternative names bisBenzimide; HO 33258; Hoechst-33258
Biological description

10mg/ml staining solution in water. Blue fluorescent DNA and nuclei stain which is frequently used in immunofluorescent work. Cell permeable but does not induce apoptosis.

Hoechst 33258 solid also available in 25mg and 100mg.

 

Description

Blue fluorescent nuclear DNA stain. 10mg/ml staining solution in water. Solid also available in 25mg and 100mg.

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Solubility & Handling

Storage instructions +4°C
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Chemical name Bisbenzimidazole trihydrochloride
Molecular Weight 533.88
Chemical structure  Chemical Structure
Molecular Formula C25H24N6O.3HCl
CAS Number 23491-45-4

References for Hoechst 33258 Staining Solution (10mg/ml)

References are publications that support the biological activity of the product
  • Binding of Hoechst 33258 and its derivatives to DNA.

    Bazhulina NP et al (2009) Journal of biomolecular structure & dynamics 26 : 701-18
  • The Hoechst 33258 covalent dimer covers a total turn of the double-stranded DNA.

    Streltsov SA et al (2006) Journal of biomolecular structure & dynamics 24 : 285-302
  • Hoechst 33258: a fluorescent nuclear counterstain suitable for double-labelling immunofluorescence.

    Bainbridge DR et al (1983) Journal of immunological methods 62 : 193-5

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