Doxycycline hyclate
(HB4608)
Product overview
| Name | Doxycycline hyclate |
| Description | Broad-spectrum antibiotic. Used in inducible Tet-on/ Tet-off gene expression systems for gene editing. |
| Alternative names | DOX |
| Purity | >93% |
| Customer comments | I received and used your DOX to induce Tet-driven expression of fluorescent protein in neuronal primary cultures. There was no evident toxicity in higher concentrations than usual. Verified customer, Uni Marburg |
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Biological Data
| Biological description | Broad-spectrum antibiotic. Tetracycline analog. Also acts as a matrix metalloprotease (MMP) inhibitor. Commonly used in the inducible Tet-on/ Tet-off gene expression system to control transcription. Recently used to develop a doxycycline-inducible, viral mediated CRISPR/Cas9 system. |
Solubility & Handling
| Storage instructions | +4°C |
| Solubility overview | Soluble in DMSO (100 mM) and water (100 mM) |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
| Chemical name | (4S,4aR,5S,5aR,6R,12aS)-4-(Dimethylamino)- 3,5,10,12,12a-pentahydroxy- 6-methyl- 1,11-dioxo- 1,4,4a,5,5a,6,11,12a-octahydrotetracene- 2-carboxamide hydrochloride hemiethanolate hemihydrate |
| Molecular Weight | 512.94 |
| Chemical structure | ![Doxycycline hyclate [24390-14-5] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb4608.png "Doxycycline hyclate [24390-14-5]") |
| Molecular Formula | C22H24N2O8.HCl.0.5H2O.0.5C2H6O |
| CAS Number | 24390-14-5 |
| PubChem identifier | 54686898 |
| SMILES | CCO.C[C@@H]1[C@H]2[C@@H]([C@H]3[C@@H](C(=O)C(=C([C@]3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.Cl |
| InChi | InChI=1S/2C22H24N2O8.C2H6O.2ClH.H2O/c2*1-7-8-5-4-6-9(25)11(8)16(26)12-10(7)17(27)14-15(24(2)3)18(28)13(21(23)31)20(30)22(14,32)19(12)29;1-2-3;;;/h2*4-7,10,14-15,17,25-27,30,32H,1-3H3,(H2,23,31);3H,2H2,1H3;2*1H;1H2/t2*7-,10+,14+,15-,17-,22-;;;;/m00..../s1 |
| InChiKey | OJMDBNRHBLXJLF-LJTDZVLQSA-N |
| MDL number | MFCD07357237 |
| Appearance | Pale yellow to yellow |
References for Doxycycline hyclate
References are publications that support the biological activity of the product
-
Inducible in vivo genome editing with CRISPR-Cas9.
Dow et al (2015) Nat Biotechnol 33(4) : 390-394 -
The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing.
de Solis et al (201 6) Front. Cell. Neurosci. 18 : 9-70 -
Doxycycline inhibits MMPs via modulation of plasminogen activators in focal cerebral ischemia.
Burggraf et al (2007) Neurobiol Dis 25(3) : 506-13
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