Doxycycline hyclate

(HB4608)
Technical documents: SDS CoA Datasheet

Product overview

Name Doxycycline hyclate
Description Broad-spectrum antibiotic. Used in inducible Tet-on/ Tet-off gene expression systems for gene editing.
Alternative names DOX
Purity >93%
Customer comments

I received and used your DOX to induce Tet-driven expression of fluorescent protein in neuronal primary cultures. There was no evident toxicity in higher concentrations than usual. Verified customer, Uni Marburg

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Biological Data

Biological description

Broad-spectrum antibiotic. Tetracycline analog. Also acts as a matrix metalloprotease (MMP) inhibitor. 

Commonly used in the inducible Tet-on/ Tet-off gene expression system to control transcription. 

Recently used to develop a doxycycline-inducible, viral mediated CRISPR/Cas9 system.

Solubility & Handling

Storage instructions +4°C
Solubility overview Soluble in DMSO (100 mM) and water (100 mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

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Dilution

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Chemical Data

Chemical name (4S,4aR,5S,5aR,6R,12aS)-4-(Dimethylamino)- 3,5,10,12,12a-pentahydroxy- 6-methyl- 1,11-dioxo- 1,4,4a,5,5a,6,11,12a-octahydrotetracene- 2-carboxamide hydrochloride hemiethanolate hemihydrate
Molecular Weight 512.94
Chemical structure Doxycycline hyclate [24390-14-5] Chemical Structure
Molecular Formula C22H24N2O8.HCl.0.5H2O.0.5C2H6O
CAS Number 24390-14-5
PubChem identifier 54686898
SMILES CCO.C[C@@H]1[C@H]2[C@@H]([C@H]3[C@@H](C(=O)C(=C([C@]3(C(=O)C2=C(C4=C1C=CC=C4O)O)O)O)C(=O)N)N(C)C)O.Cl
InChi InChI=1S/2C22H24N2O8.C2H6O.2ClH.H2O/c2*1-7-8-5-4-6-9(25)11(8)16(26)12-10(7)17(27)14-15(24(2)3)18(28)13(21(23)31)20(30)22(14,32)19(12)29;1-2-3;;;/h2*4-7,10,14-15,17,25-27,30,32H,1-3H3,(H2,23,31);3H,2H2,1H3;2*1H;1H2/t2*7-,10+,14+,15-,17-,22-;;;;/m00..../s1
InChiKey OJMDBNRHBLXJLF-LJTDZVLQSA-N
MDL number MFCD07357237
Appearance Pale yellow to yellow

References for Doxycycline hyclate

References are publications that support the biological activity of the product
  • Inducible in vivo genome editing with CRISPR-Cas9.

    Dow et al (2015) Nat Biotechnol 33(4) : 390-394
  • The Development of a Viral Mediated CRISPR/Cas9 System with Doxycycline Dependent gRNA Expression for Inducible In vitro and In vivo Genome Editing.

    de Solis et al (201 6) Front. Cell. Neurosci. 18 : 9-70
  • Doxycycline inhibits MMPs via modulation of plasminogen activators in focal cerebral ischemia.

    Burggraf et al (2007) Neurobiol Dis 25(3) : 506-13