Brefeldin A is a reversible inhibitor of protein transport.
Following treatment with Brefeldin A, the Golgi complex disassembles and redistributes into the endoplasmic reticulum within minutes. Brefeldin A is a potent, rapid and reversible inhibitor of secretion.
Brefeldin A inhibits the GTPase exchange factor acting on the ARF protein. ARF activates ADP-ribosylation factors to the golgi complex.
Uses Brefeldin A is widely used in studies of membrane trafficking. It increases intracellular cytokine staining signals and is commonly used for intracellular staining of cytokines for flow cytometry. It blocks transport processes during cell activation and causes an accumulation of cytokines at the golgi complex/ endoplasmic reticulum.
Brefeldin A also shows antibiotic actions and induces apoptosis and autophagy in mammalian cells. Recently, it has been shown to enhance CRISPR-mediated homology-directed repair (HDR) in hiPSCs (human induced pluripotent stem cells).
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
![Brefeldin A BFA [20350-15-6] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb2949_1.png "Brefeldin A BFA [20350-15-6]")
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