Product overview
| Name | Brefeldin A (BFA) |
| Description | Reversible protein transport inhibitor. Commonly used in cytokine staining. Enhances CRISPR-mediated HDR. |
| Alternative names | BFA, Synergisidin, Nectrolide, Decumbin, Cyanein |
| Purity | >98% |
Biological Data
| Biological description | Brefeldin A is a reversible inhibitor of protein transport. Following treatment with Brefeldin A, the Golgi complex disassembles and redistributes into the endoplasmic reticulum within minutes. Brefeldin A is a potent, rapid and reversible inhibitor of secretion.
|
Solubility & Handling
| Storage instructions | -20°C (desiccate) |
| Solubility overview | Soluble in DMSO (50mM) and in ethanol (10mM) |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Chemical Data
| Chemical name | 1,6,7,8,9,11aβ,12,13,14,14αa-Decahydro-1β,13α-dihydroxy-6β-methyl-4H-cyclopent(f)oxacyclotridecin-4-one |
| Molecular Weight | 280.36 |
| Chemical structure | ![Brefeldin A BFA [20350-15-6] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb2949_1.png "Brefeldin A BFA [20350-15-6]") |
| Molecular Formula | C16H24O4 |
| CAS Number | 20350-15-6 |
| PubChem identifier | 6436187 |
| SMILES | [H][C@]1(C)CCC\C=C\C2C[C@H](O)C[C@H]2[C@H](O)\C=C\C(=O)O1 |
| InChi | InChI=1S/C16H24O4/c1-11-5-3-2-4-6-12-9-13(17)10-14(12)15(18)7-8-16(19)20-11/h4,6-8,11-15,17-18H,2-3,5,9-10H2,1H3/b6-4+,8-7+/t11-,12?,13-,14+,15+/m0/s1 |
| InChiKey | KQNZDYYTLMIZCT-KFKPYADVSA-N |
| MDL number | MFCD12913297 |
| Appearance | White to off-white solid |
References for Brefeldin A (BFA)
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Small molecules enhance CRISPR genome editing in pluripotent stem cells.
Yu et al (2015) Cell Stem Cell 16(2) : 142-7 -
Molecular mechanism and functional role of brefeldin A-mediated ADP-ribosylation of CtBP1/BARS.
Colanzi et al (2013) Proc Natl Acad Sci U S A 110(24) : 9794-9 -
Brefeldin A: the advantage of being uncompetitive.
Chardin and McCormick (1999) Cell 97(2) : 153-5 -
Golgi tubule traffic and the effects of brefeldin A visualized in living cells.
Sciaky et al (1997) J Cell Biol 39(5) : 1137-55 -
Detection of intracellular cytokines by flow cytometry.
Jung et al (1993) J Immunol Methods. 159(1-2) : 197-207
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- Code:
- HB0502