Product overview

Name Brefeldin A (BFA)
Description Reversible protein transport inhibitor. Commonly used in cytokine staining. Enhances CRISPR-mediated HDR.
Alternative names BFA, Synergisidin, Nectrolide, Decumbin, Cyanein
Purity >98%
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Biological Data

Biological description

Brefeldin A is a reversible inhibitor of protein transport. 

Following treatment with Brefeldin A, the Golgi complex disassembles and redistributes into the endoplasmic reticulum within minutes. Brefeldin A is a potent, rapid and reversible inhibitor of secretion.


Brefeldin A inhibits the GTPase exchange factor acting on the ARF protein. ARF activates ADP-ribosylation factors to the golgi complex.


Uses
Brefeldin A is widely used in studies of membrane trafficking. It increases intracellular cytokine staining signals and is commonly used for intracellular staining of cytokines for flow cytometry. It blocks transport processes during cell activation and causes an accumulation of cytokines at the golgi complex/ endoplasmic reticulum.


Brefeldin A also shows antibiotic actions and induces apoptosis and autophagy in mammalian cells. Recently, it has been shown to enhance CRISPR-mediated homology-directed repair (HDR) in hiPSCs (human induced pluripotent stem cells).


Monensin sodium salt also available

Solubility & Handling

Storage instructions -20°C (desiccate)
Solubility overview Soluble in DMSO (50mM) and in ethanol (10mM)
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

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Dilution

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Chemical Data

Chemical name 1,6,7,8,9,11aβ,12,13,14,14αa-Decahydro-1β,13α-dihydroxy-6β-methyl-4H-cyclopent(f)oxacyclotridecin-4-one
Molecular Weight 280.36
Chemical structure Brefeldin A BFA [20350-15-6] Chemical Structure
Molecular Formula C16H24O4
CAS Number 20350-15-6
PubChem identifier 6436187
SMILES [H][C@]1(C)CCC\C=C\C2C[C@H](O)C[C@H]2[C@H](O)\C=C\C(=O)O1
InChi InChI=1S/C16H24O4/c1-11-5-3-2-4-6-12-9-13(17)10-14(12)15(18)7-8-16(19)20-11/h4,6-8,11-15,17-18H,2-3,5,9-10H2,1H3/b6-4+,8-7+/t11-,12?,13-,14+,15+/m0/s1
InChiKey KQNZDYYTLMIZCT-KFKPYADVSA-N
MDL number MFCD12913297
Appearance White to off-white solid

References for Brefeldin A (BFA)

References are publications that support the biological activity of the product
  • Small molecules enhance CRISPR genome editing in pluripotent stem cells.

    Yu et al (2015) Cell Stem Cell 16(2) : 142-7
  • Molecular mechanism and functional role of brefeldin A-mediated ADP-ribosylation of CtBP1/BARS.

    Colanzi et al (2013) Proc Natl Acad Sci U S A 110(24) : 9794-9
  • Brefeldin A: the advantage of being uncompetitive.

    Chardin and McCormick (1999) Cell 97(2) : 153-5
  • Golgi tubule traffic and the effects of brefeldin A visualized in living cells.

    Sciaky et al (1997) J Cell Biol 39(5) : 1137-55
  • Detection of intracellular cytokines by flow cytometry.

    Jung et al (1993) J Immunol Methods. 159(1-2) : 197-207