α-Galactosylceramide (alpha-GalCer) (KRN7000)
Product overview
Name | α-Galactosylceramide (alpha-GalCer) (KRN7000) |
Description | Immunostimulant. Natural killer T cell stimulator. |
Alternative names | α-Gal-Cer; KRN7000 |
Purity | >96% |
Images
Biological Data
Biological description | Alpha-GalCer is an immunostimulant. It is a potent stimulator of natural killer T (NKT) cells and a specific ligand of the lipid-binding MHC class I-like protein CD1d in human and mouse NKT cells. It protects against LPS-induced shock and is also a potent antitumor compound.
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Solubility & Handling
Storage instructions | -20°C |
Solubility overview | Soluble in Tween20 or PBS for cell culture use. Also Soluble in pyridine (not suitable for cell culture). Aliquot into glass vials. |
Handling | This compound is is inherently an extremely hydrophobic molecule and is insoluble in water, methanol, ethanol and other organic solvents. It is very slightly soluble in tetrahydrofuran and pyridine. Almost all methods for solubilizing this material in primarily aqueous media will contain at least some detergent and somewhat cloudy solutions may be produced which are still acceptable to use. Some example protocols are as follows:
Tween20 & PBS, Heat/Sonicate
DMSO, Heat/Sonicate
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Handling continued.. | 2 Steps: Chloroform:Methanol > Evaporation > DMSO, PBS or Tween20 > Heat > Sonicate
5.6% sucrose, 0.75% L-histidine and 0.5% Tween20 > Heat > Sonicate
Please ensure solutions are mixed well immediately before use.
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Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
Purity | >96% |
Molecular Weight | 858.3 |
Chemical structure | |
Molecular Formula | C50H99NO9 |
CAS Number | 158021-47-7 |
PubChem identifier | 354334933 |
SMILES | CCCCCCCCCCCCCCCCCCCCCCCCCC(=O)NC(CO[C@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O)[C@H](O)[C@H](O)CCCCCCCCCCCCCC |
Source | Originally isolated from the marine sponge Angelas mauritianus |
InChiKey | LSUQVAFARUJTHS-ABBGRRHVSA-N |
MDL number | MFCD00939559 |
Appearance | Off-white solid |
References for α-Galactosylceramide (alpha-GalCer) (KRN7000)
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α-Galactosylceramide stimulates splenic lymphocyte proliferation in vitro and increases antibody production in vivo in late neonatal-age mice.
Chen and Ross (2015) Clin Exp Immunol 179(2) : 188-96 -
Stimulation of invariant natural killer T cells by α-Galactosylceramide activates the JAK-STAT pathway in endothelial cells and reduces angiogenesis in the 5T33 multiple myeloma model.
Nur et al (2014) Br J Haematol 167(5) : 651-63 -
Activation of natural killer T cells by alpha-galactosylceramide in the presence of CD1d provides protection against colitis in mice.
Saubermann et al (2000) Gastroenterology 119(1) : 119-28
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Invariant Natural Killer T cells coordinate removal of senescent cells
Arora et al (2021) Med (N Y). (8) : 938-950PubMedID: 34617070