(S)-3,5-DHPG

(S)-3,5-DHPG is a widely used selective group I mGlu receptor agonist (EC₅₀ values are 2 and 6.6 µM at mGlu₅ and mGlu₁, 106 µM at mGlu₃ and >1000 µM at mGlu₂,₄,₇,₈ receptors respectively).

(S)-3,5-DHPG is the active enantiomer of DHPG. The racemic (R,S)-DHPG is also available.

(S)-3,5-DHPG induces long term depression (LTD) (mGluR/DHPG-LTD). DHPG-LTD can temporarily be reversed by other mGluR antagonists such as (S)-MCPG.

The CaMKII inhibitor KN-62 has also been shown to facilitate DHPG-LTD and (S)-3,5-DHPG-induced LTD is thought to involve rapid protein synthesis which can be antagonized by the protein synthesis inhibitor anisomycin.

(S)-3,5-DHPG is active in vivo and shows a variety of biological actions.

Figure 1: Gamma-frequency oscillations recorded in CA3 region of rat in vitro hippocampal slice preparation.

Increase in gamma frequency oscillatory activity (dark blue) following (S)-3,5-DHPG application. Under control conditions there is no gamma activity (light blue). 

Data kindly provided by Neurexpert

The compound is of high purity, however it is air sensitive and light promoted oxidation may cause slight discoloration of the compound. Product performance should not be affected, and this decolourisation can be ignored.

It has been shown that this compound rapidly decomposes when dissolved in alkaline solution. We therefore recommend that you store the solid material at -20°C and protect from light. The compound should be stable for a least 6 months.

When possible, you should make up solutions and use immediately. If storage of solutions is required, you should aliquot out the solution into tightly sealed vials and store at -20°C and store these for up to one month. Allow the product to equilibrate to RT for at least one hour before opening and using.