Thioflavin X (ThX)

Novel, next generation cell-permeable fluorescent amyloid stain for in vitro β-Amyloid Peptide (1-42) (human) staining in brain tissues. Shows 5x increase in brightness and 7x increase in binding affinity to amyloidogenic proteins to display superior photophysical and binding properties compared to Thioflavin T (ThT). Unlike Thioflavin T, Thioflavin X (ThX) can be used for monitoring structural changes of amyloid β oligomers. The improved optical properties (extinction coefficient, quantum yield and brightness) of Thioflavin X (ThX) allow monitoring of structural differences in oligomeric species which is not observable with Thioflavin T imaging. It is suitable for studying unique structural amyloid features in bulk and on a single-aggregate level and also allows detection of amyloid β-sheet species at the early stages of protein aggregation. Also suitable for use in super-resolution microscopy with ~20nm resolution.