Novel, next generation cell-permeable fluorescent amyloid stain for in vitroβ-Amyloid Peptide (1-42) (human) staining in brain tissues. Shows 5x increase in brightness and 7x increase in binding affinity to amyloidogenic proteins to display superior photophysical and binding properties compared to Thioflavin T (ThT). Unlike Thioflavin T, Thioflavin X (ThX) can be used for monitoring structural changes of amyloid β oligomers. The improved optical properties (extinction coefficient, quantum yield and brightness) of Thioflavin X (ThX) allow monitoring of structural differences in oligomeric species which is not observable with Thioflavin T imaging. It is suitable for studying unique structural amyloid features in bulk and on a single-aggregate level and also allows detection of amyloid β-sheet species at the early stages of protein aggregation. Also used to super-resolve the structures of tau aggregates (especially early aggregate species with lengths under 100-200 nm).
Suitable for use in super-resolution microscopy with ~20nm resolution.
Solubility & Handling
Storage instructions
-20°C
Solubility overview
Soluble in DMSO (100mM) and in EtOH (10 mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
References are publications that support the biological activity of the product
Cavity Lasing Characteristics of Thioflavin T and Thioflavin X in Different Solvents and Their Interaction with DNA for the Controlled Reduction of a Light Amplification Threshold in Solid-State Biofilms.
Rusakov K et al (2023) ACS applied optical materials 1 : 1922-1929
![Thioflavin X (ThX) [2683063-26-3] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb17774_1.png "Thioflavin X (ThX) [2683063-26-3]")
Understanding purity and quality - a guide for life scientists
