Novel, next generation blue-green, cell-permeable fluorescent amyloid stain. Shows 5x increase in brightness and 7x increase in binding affinity to amyloidogenic proteins (α-syn) to display superior photophysical and binding properties compared to Thioflavin T (ThT). Thioflavin X (ThX) outperforms Thioflavin T as a single-aggregate probe of β-Amyloid Peptide (1-42) (human) and P301S tau as ThX is able to detect greater numbers of fluorescent species of each than ThT.
Unlike Thioflavin T, Thioflavin X (ThX) can be used for monitoring structural changes of amyloid β oligomers. The improved optical properties (extinction coefficient, quantum yield and brightness) of Thioflavin X (ThX) allow monitoring of structural differences in oligomeric species which is not observable with Thioflavin T imaging. It is suitable for studying unique structural amyloid features in bulk and on a single-aggregate level and also allows detection of amyloid β-sheet species at the early stages of protein aggregation. Also used to super-resolve the structures of tau aggregates (especially early aggregate species with lengths under 100-200 nm). Suitable for use in super-resolution microscopy with ~20nm resolution.
Figure 1. Excitation / Emission spectra of Thioflavin X ± 100µM Aβ
Thioflavin X was dissolved at 5µM in either PBS or PBS + 100µM Amyloid Beta (HB9805) before then excitation / emission spectra were measured and signal normalized to the maximum excitation and emission for Thioflavin X + 100µM Aβ respectively.
Figure 1. Excitation / Emission spectra of Thioflavin X ± 100µM Aβ
Thioflavin X was dissolved at 5µM in either PBS or PBS + 100µM Amyloid Beta (HB9805) before then excitation / emission spectra were measured and signal normalized to the maximum excitation and emission for Thioflavin X + 100µM Aβ respectively.
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Handling
Needham et al prepared stock solutions to 10mM in DMSO. These were then diluted into 0.02µM filtered PBS (pH 7.4) to the experimentally required concentration.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
References are publications that support the biological activity of the product
Cavity Lasing Characteristics of Thioflavin T and Thioflavin X in Different Solvents and Their Interaction with DNA for the Controlled Reduction of a Light Amplification Threshold in Solid-State Biofilms.
Rusakov K et al (2023) ACS applied optical materials 1 : 1922-1929
![Thioflavin X (ThX) [2683063-26-3] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb17774_1.png "Thioflavin X (ThX) [2683063-26-3]")
Amyloid Beta Protocol (503 KB) 
