Product overview

Name Thioflavin T (ThT)
Alternative names Thioflavin T
Purity >95%
Description

Cell-permeable fluorescent amyloid stain

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Biological Data

Biological description

Cell-permeable fluorescent amyloid stain for in vitro amyloid beta staining in brain tissues. Used to detect amyloid fibrils and to study amyloid fibril structure and the mechanism by which they form. Stains insoluble senile Aβ plaques, confirms formation of β-sheet structure from mutant huntingtin exon-1 aggregates in vitro and also may also be used to monitor polyglutamine amyloid formation of tNhtt-42Q aggregates in Huntington's diseases models in vitro.

Solubility & Handling

Storage instructions -20°C
Solubility overview

Soluble in water (10 mM), and in DMSO (5 mM)

Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Purity >95%
Chemical name 2-[4-(Dimethylamino)phenyl]-3,6-dimethylbenzothiazolium chloride
Molecular Weight 318.9
Chemical structure  Chemical Structure
Molecular Formula C17H19ClN2S
CAS Number 2390-54-7
PubChem identifier 16853
SMILES CC1=CC2=C(C=C1)[N+](=C(S2)C3=CC=C(C=C3)N(C)C)C.[Cl-]
InChi InChI=1S/C17H19N2S.ClH/c1-12-5-10-15-16(11-12)20-17(19(15)4)13-6-8-14(9-7-13)18(2)3;/h5-11H,1-4H3;1H/q+1;/p-1
InChiKey JADVWWSKYZXRGX-UHFFFAOYSA-M

References for Thioflavin T (ThT)

References are publications that support the biological activity of the product
  • Thioflavin T fluoresces as excimer in highly concentrated aqueous solutions and as monomer being incorporated in amyloid fibrils.

    Sulatskaya AI et al (2017) Scientific reports 7 : 2146
  • Mechanism of thioflavin T binding to amyloid fibrils.

    Khurana R et al (2005) Journal of structural biology 151 : 229-38
  • The binding of thioflavin-T to amyloid fibrils: localisation and implications.

    Krebs MR et al (2005) Journal of structural biology 149 : 30-7

3 Item(s)