Cell-permeable fluorescent amyloid stain for in vitroamyloid beta staining in brain tissues. Used to detect amyloid fibrils and to study amyloid fibril structure and the mechanism by which they form. Stains insoluble senile Aβ plaques, confirms formation of β-sheet structure from mutant huntingtin exon-1 aggregates in vitro and may also be used to monitor polyglutamine amyloid formation of tNhtt-42Q aggregates in Huntington's diseases models in vitro.
Figure 1. Excitation / Emission spectra of Thioflavin T ± 100µM Aβ
Thioflavin T was dissolved at 5µM in either PBS or PBS + 100µM Amyloid Beta (HB9805) before then excitation / emission spectra were measured and signal normalized to the maximum excitation and emission for Thioflavin T + 100µM Aβ respectively.
Figure 1. Excitation / Emission spectra of Thioflavin T ± 100µM Aβ
Thioflavin T was dissolved at 5µM in either PBS or PBS + 100µM Amyloid Beta (HB9805) before then excitation / emission spectra were measured and signal normalized to the maximum excitation and emission for Thioflavin T + 100µM Aβ respectively.
Optical Data
Fluorescence spectra
Max excitation wavelength
Switches from 385nm to 450nm when bound to amyloid beta fibrils
Max emission wavelength
Switches from 445nm to 485nm when bound to amyloid beta fibrils
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
Amyloid Beta Protocol (368.6 KB) 
