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Biological Data
| Application notes | The biological activity was determined using a chemotaxis bioassay using human neutrophils at a concentration range of 10-100 ng/ml |
Solubility & Handling
| Solubility overview | To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100μg/ml, which can then be diluted to make a working solution |
| Handling |
- Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
- Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
- Freeze-thaw cycles should be prevented.
|
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
| Appearance | White lyophilized powder (sterile filtered & freeze-dried) |
| Formulation | Lyophilized from a 0.2μm filtered solution in PBS (pH 7.4) |
References for Recombinant rat Midkine protein
References are publications that support the biological activity of the product
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Midkine: a promising molecule for drug development to treat diseases of the central nervous system
Muramatsu T (2011) Curr Pharm Des 17(5) : 410-23 -
Astroglial neurotrophic and neurite-promoting factors
Muller HW et al (1995) Pharmacol Ther 65(1) : 43101 -
Laminin is associated with the neurite outgrowth-promoting factors" found in conditioned media"
Lander AD et al (1985) Proc Natl Acad Sci U S A 82(7) : 2183-7
Rat Midkine recombinant protein
