Recombinant rat GDNF protein

(HB1368)
Technical documents: Datasheet

Product overview

Name Recombinant rat GDNF protein
Alternative names Recombinant Rat Glial-Derived Neurotrophic Factor, ATF1, ATF2, HFB1-GDNF, GDNF.
Purity >98%
Description Rat GDNF recombinant protein
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Biological Data

Application notes Fully biologically active when compared to standard. ED50 = <0.2ng/ml (determined by a cell proliferation assay using rat C6 cells), corresponding to a specific activity of more than 5,000,000IU/mg.

Solubility & Handling

Solubility overview To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100µg/ml, which can then be diluted to make a working solution
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >98%
Source E. Coli.
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized from a sterile solution containing PBS (pH 7.4)

References for Recombinant rat GDNF protein

References are publications that support the biological activity of the product
  • Biology of GDNF and its receptors - Relevance for disorders of the central nervous system

    Ibanez CF et al (2017) Neurobiol Dis 97(Pt B) : 80-89
  • Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM)

    Euteneuer S et al (2013) Mol Cell Neurosci 54 : 30-43
  • Glial cell line-derived neurotrophic factor (GDNF): a drug candidate for the treatment of Parkinson's disease

    Grondin R et al (1998) J Neurol 245(11 Suppl 3) : P35-42

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