Recombinant rat GDNF protein

(HB1368)
Technical documents: Datasheet

Product overview

Name Recombinant rat GDNF protein
Alternative names Recombinant Rat Glial-Derived Neurotrophic Factor, ATF1, ATF2, HFB1-GDNF, GDNF.
Purity >98%
Description Rat GDNF recombinant protein
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Biological Data

Application notes Fully biologically active when compared to standard. ED50 = <0.2ng/ml (determined by a cell proliferation assay using rat C6 cells), corresponding to a specific activity of more than 5,000,000IU/mg.

Solubility & Handling

Solubility overview To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100µg/ml, which can then be diluted to make a working solution
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >98%
UniProt ID Q07731
Source E. Coli.
Structure image  Chemical Structure
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized from a sterile solution containing PBS (pH 7.4)

References for Recombinant rat GDNF protein

References are publications that support the biological activity of the product
  • Biology of GDNF and its receptors - Relevance for disorders of the central nervous system

    Ibanez CF et al (2017) Neurobiol Dis 97(Pt B) : 80-89
  • Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM)

    Euteneuer S et al (2013) Mol Cell Neurosci 54 : 30-43
  • Glial cell line-derived neurotrophic factor (GDNF): a drug candidate for the treatment of Parkinson's disease

    Grondin R et al (1998) J Neurol 245(11 Suppl 3) : P35-42

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