Recombinant mouse NT-3 protein

(HB9400)
Technical documents: CoA Datasheet

Product overview

Name Recombinant mouse NT-3 protein
Alternative names Recombinant Mouse Neurotrophin-3, Neurotrophic factor, Nerve growth factor-2, NGF-2, HDNF, NT-3, Neurotrophin-3, Ntf3, Ntf-3, AI316846, AI835689, Nt3.
Purity >97%
Description Recombinant mouse Neurotrophin-3 protein
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Biological Data

Application notes The ED50 = ˜ 1-10 ng/ml (determined by the dose-dependent proliferation of BaF3 cells transfected with TrkB receptor), corresponding to a specific activity of 100,000-1,000,000 units/mg

Solubility & Handling

Storage instructions -20°C
Solubility overview To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100μg/ml, which can then be diluted to make a working solution
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Purity >97%
Molecular Weight 27.5
Source E. Coli.
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized from 0.02% TFA

References for Recombinant mouse NT-3 protein

References are publications that support the biological activity of the product
  • Neurotrophin-3 (NT-3) modulates early differentiation of oligodendrocytes in rat brain cortical cultures

    Heinrich M et al (1999) Glia 28(3) : 244-55
  • NT-3, like NGF, is required for survival of sympathetic neurons, but not their precursors

    Francis N et al (1999) Dev Biol 210(2) : 411-27
  • Early BDNF, NT-3, and NT-4 signaling events

    Yuen EC et al (1999) Exp Neurol 159(1) : 297-308

3 Item(s)