Recombinant mouse NT-3 protein

(HB9400)
Technical documents: CoA Datasheet

Product overview

Name Recombinant mouse NT-3 protein
Alternative names Recombinant Mouse Neurotrophin-3, Neurotrophic factor, Nerve growth factor-2, NGF-2, HDNF, NT-3, Neurotrophin-3, Ntf3, Ntf-3, AI316846, AI835689, Nt3.
Purity >97%
Description Recombinant mouse Neurotrophin-3 protein
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Biological Data

Application notes The ED50 = ˜ 1-10 ng/ml (determined by the dose-dependent proliferation of BaF3 cells transfected with TrkB receptor), corresponding to a specific activity of 100,000-1,000,000 units/mg

Solubility & Handling

Storage instructions -20°C
Solubility overview To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100μg/ml, which can then be diluted to make a working solution
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Purity >97%
UniProt ID P20181
Molecular Weight 27.5
Source E. Coli.
Structure image  Chemical Structure
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized from 0.02% TFA

References for Recombinant mouse NT-3 protein

References are publications that support the biological activity of the product
  • Neurotrophin-3 (NT-3) modulates early differentiation of oligodendrocytes in rat brain cortical cultures

    Heinrich M et al (1999) Glia 28(3) : 244-55
  • NT-3, like NGF, is required for survival of sympathetic neurons, but not their precursors

    Francis N et al (1999) Dev Biol 210(2) : 411-27
  • Early BDNF, NT-3, and NT-4 signaling events

    Yuen EC et al (1999) Exp Neurol 159(1) : 297-308

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