˜20-50 ng/ml (determined dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures)
Solubility & Handling
Solubility overview
To make a working stock solution, add deionized water to make a solution (0.5mg/mL) and allow the lyophilized material to dissolve. Filter the product using an appropriate sterile filter before using it in cell culture
Handling
Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use