Recombinant human NT-4 protein

(HB8843)
Technical documents: Datasheet

Product overview

Name Recombinant human NT-4 protein
Alternative names Recombinant Human Neurotrophin-4, NT4, NT5, NTF5, NT-4/5, NTF4, Neurotrophin-4, Neutrophic factor 4, Neurotrophin-5, NT-5.
Purity >97%
Description Recombinant mouse Neurotrophin-4 protein
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Biological Data

Application notes ˜20-50 ng/ml (determined dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures)

Solubility & Handling

Solubility overview To make a working stock solution, add deionized water to make a solution (0.5mg/mL) and allow the lyophilized material to dissolve. Filter the product using an appropriate sterile filter before using it in cell culture
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Purity >97%
UniProt ID P34130
Source E. Coli.
Structure image  Chemical Structure
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized from a solution (1mg/ml) in water containing phosphate buffer (20mM, pH7.4) and NaCl (150mM)

References for Recombinant human NT-4 protein

References are publications that support the biological activity of the product
  • NT-4 protein is localized in neuronal cells in the brain stem as well as the dorsal root ganglion of embryonic and adult rats

    Katoh-Semba R et al (2003) J Neurochem 86(3) : 660-8
  • A new role for neurotrophins: involvement of brain-derived neurotrophic factor and neurotrophin-4 in hair cycle control

    Botchkarev VA et al (1999) FASEB J 13(2) : 395-410
  • Neurotrophin-4/5 (NT-4/5) and brain-derived neurotrophic factor (BDNF) act at later stages of cerebellar granule cell differentiation

    Gao WQ et al (1995) J Neurosci 15(4) : 2656-67

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