Recombinant human NT-4 protein

(HB8843)
Technical documents: Datasheet

Product overview

Name Recombinant human NT-4 protein
Alternative names Recombinant Human Neurotrophin-4, NT4, NT5, NTF5, NT-4/5, NTF4, Neurotrophin-4, Neutrophic factor 4, Neurotrophin-5, NT-5.
Purity >97%
Description Recombinant mouse Neurotrophin-4 protein
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Biological Data

Application notes ˜20-50 ng/ml (determined dose-dependent induction of choline acetyl transferase activity in rat basal forebrain primary septal cell cultures)

Solubility & Handling

Solubility overview To make a working stock solution, add deionized water to make a solution (0.5mg/mL) and allow the lyophilized material to dissolve. Filter the product using an appropriate sterile filter before using it in cell culture
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Purity >97%
Source E. Coli.
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized from a solution (1mg/ml) in water containing phosphate buffer (20mM, pH7.4) and NaCl (150mM)

References for Recombinant human NT-4 protein

References are publications that support the biological activity of the product
  • NT-4 protein is localized in neuronal cells in the brain stem as well as the dorsal root ganglion of embryonic and adult rats

    Katoh-Semba R et al (2003) J Neurochem 86(3) : 660-8
  • A new role for neurotrophins: involvement of brain-derived neurotrophic factor and neurotrophin-4 in hair cycle control

    Botchkarev VA et al (1999) FASEB J 13(2) : 395-410
  • Neurotrophin-4/5 (NT-4/5) and brain-derived neurotrophic factor (BDNF) act at later stages of cerebellar granule cell differentiation

    Gao WQ et al (1995) J Neurosci 15(4) : 2656-67

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