Recombinant human NNT-1 protein

(HB9446)
Technical documents: Datasheet

Product overview

Name Recombinant human NNT-1 protein
Biological description

Cardiotrophin-like cytokine (CLC/ NNT-1) that belongs to the IL-6 family of cytokines.

NNT1 is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs and an inducer of IgE synthesis and B cell proliferation.

Alternative names Neurotrophin-1 Human Recombinant, Cardiotrophin-Like Cytokine Factor 1, Novel Neurotrophin-1 , BSF3, CLC, CRLF1 Associated Cytokine-Like Factor 1, B-Cell Stimulating Factor 3, B-Cell-Stimulating Factor 3, BSF-3, NNT-1, NNT1, Neurotrophin-1/B-Cell Stimulating Factor-3, Cold-Induced Sweating Syndrome 2, B-Cell Stimulatory Factor 3, CISS2, NR6, CLCF1.
Purity >95%
Description Human recombinant Neurotrophin-1 protein
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Biological Data

Application notes The ED50 = ≤15ng/ml (as measured in a cell proliferation assay using TF-1 cells transfected with human CNTF-R)

Solubility & Handling

Solubility overview To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 0.5mg/ml, which can then be diluted to make a working solution
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Purity >95%
UniProt ID Q9UBD9
Source E. Coli.
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized from a 0.2μm filtered solution in acetonitrile and TFA

References for Recombinant human NNT-1 protein

References are publications that support the biological activity of the product
  • Novel neurotrophin-1/B cell-stimulating factor-3: a cytokine of the IL-6 family

    Senaldi G et al (1999) Proc Natl Acad Sci U S A 96(20) : 11458-63
  • Characterization of recombinant human brain-derived neurotrophic factor variants

    Sunasara KM et al (1999) Arch Biochem Biophys 372(2) : 248-60
  • Pan-neurotrophin 1: a genetically engineered neurotrophic factor displaying multiple specificities in peripheral neurons in vitro and in vivo

    Ilag LL et al (1995) Proc Natl Acad Sci U S A 92(2) : 607-11

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