Recombinant human NNT-1 protein

(HB9446)
Technical documents: Datasheet

Product overview

Name Recombinant human NNT-1 protein
Biological description

Cardiotrophin-like cytokine (CLC/ NNT-1) that belongs to the IL-6 family of cytokines.

NNT1 is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs and an inducer of IgE synthesis and B cell proliferation.

Alternative names Neurotrophin-1 Human Recombinant, Cardiotrophin-Like Cytokine Factor 1, Novel Neurotrophin-1 , BSF3, CLC, CRLF1 Associated Cytokine-Like Factor 1, B-Cell Stimulating Factor 3, B-Cell-Stimulating Factor 3, BSF-3, NNT-1, NNT1, Neurotrophin-1/B-Cell Stimulating Factor-3, Cold-Induced Sweating Syndrome 2, B-Cell Stimulatory Factor 3, CISS2, NR6, CLCF1.
Purity >95%
Description Human recombinant Neurotrophin-1 protein
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Biological Data

Application notes The ED50 = ≤15ng/ml (as measured in a cell proliferation assay using TF-1 cells transfected with human CNTF-R)

Solubility & Handling

Solubility overview To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 0.5mg/ml, which can then be diluted to make a working solution
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Purity >95%
Source E. Coli.
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized from a 0.2μm filtered solution in acetonitrile and TFA

References for Recombinant human NNT-1 protein

References are publications that support the biological activity of the product
  • Novel neurotrophin-1/B cell-stimulating factor-3: a cytokine of the IL-6 family

    Senaldi G et al (1999) Proc Natl Acad Sci U S A 96(20) : 11458-63
  • Characterization of recombinant human brain-derived neurotrophic factor variants

    Sunasara KM et al (1999) Arch Biochem Biophys 372(2) : 248-60
  • Pan-neurotrophin 1: a genetically engineered neurotrophic factor displaying multiple specificities in peripheral neurons in vitro and in vivo

    Ilag LL et al (1995) Proc Natl Acad Sci U S A 92(2) : 607-11

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