Recombinant human Midkine protein

(HB7169)
Technical documents: Datasheet

Product overview

Name Recombinant human Midkine protein
Biological description

Heparin-binding cytokine that promotes growth, survival, migration and other activities of target cells.

Alternative names Recombinant Human Midkine, NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2.
Purity >95%
Description Heparin-binding growth factor
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Biological Data

Application notes Determined by its ability to chemoattract human neutrophils using a concentration range of 0.1-10 ng/ml corresponding to a specific activity of 100,000-10,000,000IU/mg.

Solubility & Handling

Solubility overview To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100μg/ml, which can then be diluted to make a working solution
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >95%
Source E. Coli.
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4

References for Recombinant human Midkine protein

References are publications that support the biological activity of the product
  • Midkine: a promising molecule for drug development to treat diseases of the central nervous system

    Muramatsu T (2011) Curr Pharm Des 17(5) : 410-23
  • Astroglial neurotrophic and neurite-promoting factors

    Muller HW et al (1995) Pharmacol Ther 65(1) : 43101
  • Laminin is associated with the neurite outgrowth-promoting factors" found in conditioned media"

    Lander AD et al (1985) Proc Natl Acad Sci U S A 82(7) : 2183-7

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