Recombinant human Midkine protein

(HB7169)
Technical documents: Datasheet

Product overview

Name Recombinant human Midkine protein
Biological description

Heparin-binding cytokine that promotes growth, survival, migration and other activities of target cells.

Alternative names Recombinant Human Midkine, NEGF-2, Neurite Growth-Promoting Factor 2, MK, Neurite outgrowth-promoting protein, Midgestation and kidney protein, Amphiregulin-associated protein, ARAP, Neurite outgrowth-promoting factor 2, FLJ27379, Midkine, MK1, NEGF2.
Purity >95%
Description Heparin-binding growth factor
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Biological Data

Application notes Determined by its ability to chemoattract human neutrophils using a concentration range of 0.1-10 ng/ml corresponding to a specific activity of 100,000-10,000,000IU/mg.

Solubility & Handling

Solubility overview To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100μg/ml, which can then be diluted to make a working solution
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >95%
UniProt ID P21741
Source E. Coli.
Structure image  Chemical Structure
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4

References for Recombinant human Midkine protein

References are publications that support the biological activity of the product
  • Midkine: a promising molecule for drug development to treat diseases of the central nervous system

    Muramatsu T (2011) Curr Pharm Des 17(5) : 410-23
  • Astroglial neurotrophic and neurite-promoting factors

    Muller HW et al (1995) Pharmacol Ther 65(1) : 43101
  • Laminin is associated with the neurite outgrowth-promoting factors" found in conditioned media"

    Lander AD et al (1985) Proc Natl Acad Sci U S A 82(7) : 2183-7

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