Recombinant human MANF protein

(HB6750)
Technical documents: Datasheet

Product overview

Name Recombinant human MANF protein
Alternative names Recombinant Human Mesencephalic Astrocyte-Derived Neurotrophic Factor, Mesencephalic astrocyte-derived neurotrophic factor, Protein ARMET, ARP, arginine-rich mutated in early stage tumors, Arginine-rich protein.
Purity >95%
Description Recombinant Human Mesencephalic Astrocyte-Derived Neurotrophic Factor protein
Write Your Own Review
You're reviewing:Recombinant human MANF protein
Rate this item:

Biological Data

Application notes The ED50 = ˜15-25micro;g/ml (determined by its ability to stimulate the proliferation of rat C6 cells)

Solubility & Handling

Solubility overview To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100μg/ml, which can then be diluted to make a working solution
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

=
x
x
More Info

Dilution

x
=
x
More Info

Chemical Data

Purity >95%
UniProt ID P55145
Source E. Coli.
Structure image  Chemical Structure
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized & filtered (0.2μm) from a solution (1mg/ml) in PBS (pH7.4)

References for Recombinant human MANF protein

References are publications that support the biological activity of the product
  • Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Elevates Stimulus-Evoked Release of Dopamine in Freely-Moving Rats

    Renko JM et al (2018) Mol Neurobiol 55(8) : 6755-6768
  • Mesencephalic astrocyte-derived neurotrophic factor is neurorestorative in rat model of Parkinson's disease

    Voutilainen MH et al (2009) J Neurosci 29(30) : 9651-9
  • MANF: a new mesencephalic, astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons

    Petrova P et al (2003) J Mol Neurosci 20(2) : 173-88

3 Item(s)