Recombinant human MANF protein

(HB6750)
Technical documents: Datasheet

Product overview

Name Recombinant human MANF protein
Alternative names Recombinant Human Mesencephalic Astrocyte-Derived Neurotrophic Factor, Mesencephalic astrocyte-derived neurotrophic factor, Protein ARMET, ARP, arginine-rich mutated in early stage tumors, Arginine-rich protein.
Purity >95%
Description Recombinant Human Mesencephalic Astrocyte-Derived Neurotrophic Factor protein
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Biological Data

Application notes The ED50 = ˜15-25micro;g/ml (determined by its ability to stimulate the proliferation of rat C6 cells)

Solubility & Handling

Solubility overview To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100μg/ml, which can then be diluted to make a working solution
Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >95%
Source E. Coli.
Appearance White lyophilized powder (sterile filtered & freeze-dried)
Formulation Lyophilized & filtered (0.2μm) from a solution (1mg/ml) in PBS (pH7.4)

References for Recombinant human MANF protein

References are publications that support the biological activity of the product
  • Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Elevates Stimulus-Evoked Release of Dopamine in Freely-Moving Rats

    Renko JM et al (2018) Mol Neurobiol 55(8) : 6755-6768
  • Mesencephalic astrocyte-derived neurotrophic factor is neurorestorative in rat model of Parkinson's disease

    Voutilainen MH et al (2009) J Neurosci 29(30) : 9651-9
  • MANF: a new mesencephalic, astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons

    Petrova P et al (2003) J Mol Neurosci 20(2) : 173-88

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