Recombinant human CNTF (His Tag) active protein

(HB7414)
Technical documents: Datasheet

Product overview

Name Recombinant human CNTF (His Tag) active protein
Alternative names Recombinant Human Ciliary Neurotrophic Factor, His Tag Active, Ciliary neurotrophic factor, CNTF, HCNTF.
Purity >95%
Description His tag active recombinant human BDNF protein
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Biological Data

Application notes The ED50 = ≤1µg/ml (for the effect measured in a cell proliferation assay using TF-1 human erythroleukemic cells)

Solubility & Handling

Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >95%
UniProt ID P26441
Source E. Coli.
Structure image  Chemical Structure
Appearance Clear solution (sterile filtered)
Formulation Solution (1mg/ml) containing Tris-HCl buffer (20 mM, pH 8.5), DTT (1mM), 30% Glycerol and 200mM NaCl

References for Recombinant human CNTF (His Tag) active protein

References are publications that support the biological activity of the product
  • Ciliary neurotrophic factor (CNTF): New facets of an old molecule for treating neurodegenerative and metabolic syndrome pathologies

    Pasquin S et al (2015) Cytokine Growth Factor Rev 26(5) : 507-15
  • Ciliary neurotrophic factor (CNTF) promotes skeletal muscle progenitor cell (MPC) viability via the phosphatidylinositol 3-kinase-Akt pathway

    Hiatt K et al (2014) J Tissue Eng Regen Med 8(12) : 963-8
  • The ciliary neurotrophic factor and its receptor, CNTFR alpha

    Sleeman MW et al (2000) Pharm Acta Helv 74(2-3) : 265-72

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