Recombinant human CNTF (His Tag) active protein

(HB7414)
Technical documents: Datasheet

Product overview

Name Recombinant human CNTF (His Tag) active protein
Alternative names Recombinant Human Ciliary Neurotrophic Factor, His Tag Active, Ciliary neurotrophic factor, CNTF, HCNTF.
Purity >95%
Description His tag active recombinant human BDNF protein
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Biological Data

Application notes The ED50 = ≤1µg/ml (for the effect measured in a cell proliferation assay using TF-1 human erythroleukemic cells)

Solubility & Handling

Handling
  • Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
  • Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
  • For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >95%
Source E. Coli.
Appearance Clear solution (sterile filtered)
Formulation Solution (1mg/ml) containing Tris-HCl buffer (20 mM, pH 8.5), DTT (1mM), 30% Glycerol and 200mM NaCl

References for Recombinant human CNTF (His Tag) active protein

References are publications that support the biological activity of the product
  • Ciliary neurotrophic factor (CNTF): New facets of an old molecule for treating neurodegenerative and metabolic syndrome pathologies

    Pasquin S et al (2015) Cytokine Growth Factor Rev 26(5) : 507-15
  • Ciliary neurotrophic factor (CNTF) promotes skeletal muscle progenitor cell (MPC) viability via the phosphatidylinositol 3-kinase-Akt pathway

    Hiatt K et al (2014) J Tissue Eng Regen Med 8(12) : 963-8
  • The ciliary neurotrophic factor and its receptor, CNTFR alpha

    Sleeman MW et al (2000) Pharm Acta Helv 74(2-3) : 265-72

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