ED50 = < 1.0 ng/ml (calculated by its ability to stimulate chick E9 DRG neurite outgrowth), corresponding to a specific activity of > 1 x 106 units/mg.
Solubility & Handling
Storage instructions
-20°C
Solubility overview
To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100μg/ml, which can then be diluted to make a working solution
Handling
Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Studies on the expression of the beta nerve growth factor (NGF) gene in the central nervous system: level and regional distribution of NGF mRNA suggest that NGF functions as a trophic factor for several distinct populations of neurons
Shelton DL et al (1986) Proc Natl Acad Sci U S A 83(8) : 2714-8
Studies on the regulation of beta-nerve growth factor gene expression in the rat iris: the level of mRNA-encoding nerve growth factor is increased in irises placed in explant cultures in vitro, but not in irises deprived of sensory or sympathetic innervat
Shelton DL et al (1986) J Cell Biol 102(5) : 1940-8
