˜4-8ng/ml (determined by the dose-dependent proliferation of the SH-SY5Y cell line). The activity can also be determined by its ability to promote survival and neurite outgrowth.
Solubility & Handling
Solubility overview
To make a stock solution, reconstitute in sterile 18MΩcm water at a concentration > 100μg/ml, which can then be diluted to make a working solution
Handling
Solutions should be made in sterile deionized water (not less than 100 µg/ml). This solution can then be further diluted with other aqueous solutions.
Following reconstitution, solutions may be stored at 4°C and are useable for around 2-7 days and for future use store at -18°C.
For long term storage, a carrier protein (0.1% HSA or BSA) should be added to stock solutions. Solutions should be aliquoted into tightly sealed vials for storage at -20°C. Freeze-thaw cycles should be prevented.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
