Ponceau S Staining Solution

Ponceau Staining Protocol

1. Run a SDS-PAGE gel and transfer to either a nitrocellulose or PVDF membrane following standard protocols (see our Western blotting protocol),
2. Following transfer, submerge the membrane in Ponceau S staining solution and incubate for 5-15 minutes with gentle agitation
3. Decant the Ponceau S stain then gently rinse the membrane with multiple changes of dH₂O until clear bands are visible. Be careful not to overrinse as this can remove the bands.
4. Image the blot or use the visible bands to cut the membrane as desired
5. Remove the stain using multiple washes of PBST / TBST until no more red remains
6. Proceed with blocking and probing following standard protocols

4°C