Product overview
| Name | Picro Sirius Red Stain Kit |
| Alternative names | PSR Stain Kit, Picrosirius Red Stain Kit |
| Biological description | Overview The picro sirius red staining technique is frequently used to histologically stain collagen I and III fibers. This kit can be used to identify fibrillar collagen networks in tissue sections. When viewed under linear polarized light, collagens appear as green, red, orange or yellow fibers. Sample orientation under linear polarized light is thought to affect the hue and signal strength of the technique. Circular polarized light may be used to overcome this limitation but requires specialized equipment. Alternatively, fluorescent imaging of picro sirius red stained samples may be used which yields a strong red fluorescent signal that is sensitive and specific for collagen and is unaffected by sample orientation. Components
The following components are required to perform this assay but are not included in the kit:
Please see our protocol booklet: Picro Sirius Red Staining Kit Protocol |
| Description | Kit for histological staining of Collagen I and III fibers |
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Images
Figure. 1: Picro Sirius red staining in the forelimb of a lamb.
Picro Sirius red staining in the forelimb of a lamb. Picro Sirius red from Hello Bio stains collagen fibres red while muscle fibres are stained yellow. For protocol see notes below
Figure. 2: Picro Sirius red staining in toad skin under both standard widefield illumination and polarised light.
Picro Sirius red staining in toad skin under both standard widefield illumination and polarised light. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and muscle fibres yellow. Under polarised light it is possible to differentiate between collagen types with collagen 1 showing as orange/red while collagen 3 is green. For protocol see notes below.
Figure. 3: Picro Sirius red staining in the forelimb of a lamb.
Picro Sirius red staining in the forelimb of a lamb. Picro Sirius red from Hello Bio stains collagen fibres red while muscle fibres are stained yellow. For protocol see notes below.
Figure. 4: Picro Sirius red staining in chimpanzee aorta.
Picro Sirius red staining in chimpanzee aorta. Picro Sirius red from Hello Bio stains collagen I and III fibres red. For protocol see notes below.
Figure. 5: Picro Sirius red staining in toad skin.
Pirco Sirius red staining in toad skin. Under widefield illuminiation Picro Sirius red from Hello Bio stains collagen fibres red wheread muscle fibres and the cytosol of cells show as yellow. For protocol see notes below.
Figure. 6: Picro Sirius red staining in chimpanzee testicle.
Picro Sirius red staining in chimpanzee testicle. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and the cytosol of cells yellow. For protocol see notes below.
Figure. 7: Picro Sirius red staining in chimpanzee liver.
Picro Sirius red staining in Chimpanzee liver. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and the cytosol of cells yellow. For protocol see notes below.
Figure. 8: Picro Sirius red staining in kitten heart.
Picro Sirius red staining in kitten heart. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and muscle fibres yellow. For protocol see notes below
Figure. 9: Picro Sirius red staining in kidney tissue.
Picro Sirius red staining in kidney tissue. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and cytosol of cells yellow. For protocol see notes below
Figure 10. Picro Sirius red staining in formalin fixed Chimpanzee aorta
Picro Sirius red staining in formalin fixed Chimpanzee aorta under both standard widefield illumination and polarised light. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and muscle fibres yellow. Under polarised light it is possible to differentiate between collagen types with collagen 1 showing as orange/red while collagen 3 is green. For protocol see notes below.
Figure. 11: Picro Sirius red staining in formalin fixed Chimpanzee aorta under both standard widefield illumination and polarised light.
Picro Sirius red staining in formalin fixed Chimpanzee aorta under both standard widefield illumination and polarised light. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and muscle fibres yellow. Under polarised light it is possible to differentiate between collagen types with collagen 1 showing as orange/red while collagen 3 is green. For protocol see notes below.
Figure. 12: Picro Sirius red staining in Zenker fixed juvenile cat heart under both standard widefield illumination and polarised light.
Picro Sirius red staining in Zenker fixed juvenile cat heart under both standard widefield illumination and polarised light. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and muscle fibres yellow. Under polarised light it is possible to differentiate between collagen types with collagen 1 showing as orange/red while collagen 3 is green. For protocol see notes below.
Figure. 13: Picro Sirius red staining in Bouins fixed Chimpanzee testis under both standard widefield illumination and polarised light.
Picro Sirius red staining in Bouins fixed Chimpanzee testis under both Standard 1 widefield illumination and polarised light. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and muscle fibres yellow. Under polarised light it is possible to differentiate between collagen types with collagen 1 showing as orange/red while collagen 3 is green. For protocol see notes below.
Figure. 14: Picro Sirius red staining in bouins fixed Chimpanzee testis.
Picro Sirius red staining in Bouins fixes Chimpanzee testis. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and the cytosol of cells yellow. For protocol see notes below
Figure. 1: Picro Sirius red staining in the forelimb of a lamb.
Picro Sirius red staining in the forelimb of a lamb. Picro Sirius red from Hello Bio stains collagen fibres red while muscle fibres are stained yellow. For protocol see notes below
Figure. 2: Picro Sirius red staining in toad skin under both standard widefield illumination and polarised light.
Picro Sirius red staining in toad skin under both standard widefield illumination and polarised light. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and muscle fibres yellow. Under polarised light it is possible to differentiate between collagen types with collagen 1 showing as orange/red while collagen 3 is green. For protocol see notes below.
Figure. 3: Picro Sirius red staining in the forelimb of a lamb.
Picro Sirius red staining in the forelimb of a lamb. Picro Sirius red from Hello Bio stains collagen fibres red while muscle fibres are stained yellow. For protocol see notes below.
Figure. 4: Picro Sirius red staining in chimpanzee aorta.
Picro Sirius red staining in chimpanzee aorta. Picro Sirius red from Hello Bio stains collagen I and III fibres red. For protocol see notes below.
Figure. 5: Picro Sirius red staining in toad skin.
Pirco Sirius red staining in toad skin. Under widefield illuminiation Picro Sirius red from Hello Bio stains collagen fibres red wheread muscle fibres and the cytosol of cells show as yellow. For protocol see notes below.
Figure. 6: Picro Sirius red staining in chimpanzee testicle.
Picro Sirius red staining in chimpanzee testicle. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and the cytosol of cells yellow. For protocol see notes below.
Figure. 7: Picro Sirius red staining in chimpanzee liver.
Picro Sirius red staining in Chimpanzee liver. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and the cytosol of cells yellow. For protocol see notes below.
Figure. 8: Picro Sirius red staining in kitten heart.
Picro Sirius red staining in kitten heart. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and muscle fibres yellow. For protocol see notes below
Figure. 9: Picro Sirius red staining in kidney tissue.
Picro Sirius red staining in kidney tissue. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and cytosol of cells yellow. For protocol see notes below
Figure 10. Picro Sirius red staining in formalin fixed Chimpanzee aorta
Picro Sirius red staining in formalin fixed Chimpanzee aorta under both standard widefield illumination and polarised light. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and muscle fibres yellow. Under polarised light it is possible to differentiate between collagen types with collagen 1 showing as orange/red while collagen 3 is green. For protocol see notes below.
Figure. 11: Picro Sirius red staining in formalin fixed Chimpanzee aorta under both standard widefield illumination and polarised light.
Picro Sirius red staining in formalin fixed Chimpanzee aorta under both standard widefield illumination and polarised light. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and muscle fibres yellow. Under polarised light it is possible to differentiate between collagen types with collagen 1 showing as orange/red while collagen 3 is green. For protocol see notes below.
Figure. 12: Picro Sirius red staining in Zenker fixed juvenile cat heart under both standard widefield illumination and polarised light.
Picro Sirius red staining in Zenker fixed juvenile cat heart under both standard widefield illumination and polarised light. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and muscle fibres yellow. Under polarised light it is possible to differentiate between collagen types with collagen 1 showing as orange/red while collagen 3 is green. For protocol see notes below.
Figure. 13: Picro Sirius red staining in Bouins fixed Chimpanzee testis under both standard widefield illumination and polarised light.
Picro Sirius red staining in Bouins fixed Chimpanzee testis under both Standard 1 widefield illumination and polarised light. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and muscle fibres yellow. Under polarised light it is possible to differentiate between collagen types with collagen 1 showing as orange/red while collagen 3 is green. For protocol see notes below.
Figure. 14: Picro Sirius red staining in bouins fixed Chimpanzee testis.
Picro Sirius red staining in Bouins fixes Chimpanzee testis. Under widefield illumination Picro Sirius red from Hello Bio stains collagen fibres red and the cytosol of cells yellow. For protocol see notes below
Biological Data
| Application notes | Please see our protocol booklet: Picro Sirius Red Staining Kit Protocol Protocol summary:
Please see our protocol booklet: Picro Sirius Red Staining Kit Protocol Interpretation of staining / results: Polarized Light Microscopy:
Light Microscopy:
Control tissue: Lung, Muscle, Kidney or Uterus may be used as a control tissue. |
Solubility & Handling
| Storage instructions | Room temperature |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Technical guides
Understanding purity and quality - a guide for life scientistsReferences for Picro Sirius Red Stain Kit
References are publications that support the biological activity of the product
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Method for Picrosirius Red-Polarization Detection of Collagen Fibers in Tissue Sections
Rittie (2017) Methods Mol Biol 1627 : 395-407 -
Picrosirius Red Staining: A Useful Tool to Appraise Collagen Networks in Normal and Pathological Tissues
Lattouf et al (2014) J Histochem Cytochem 62(10) : 751-8 -
Picrosirius Staining Plus Polarization Microscopy, a Specific Method for Collagen Detection in Tissue Sections
Junqueria et al (1979) Histochem J 11(4) : 447-55
Publications
These publications cite the use of Picro Sirius Red Stain Kit purchased from Hello Bio:
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Bevacizumab, a vascular endothelial growth factor inhibitor, promotes orthodontic tooth movement in an experimental rat model
Aboushashish et al (2023) Heliyon : e16217 -
Angiotensin(1-7) attenuates tooth movement and regulates alveolar bone response during orthodontic force application in experimental animal model.
Abuohashish H et al (2023) Progress in orthodontics 24 : 33PubMedID: 37840086
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