Nile Red

(HB0810)

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Product overview

Name Nile Red
Alternative names Nile blue A oxazone
Biological description Photostable polarity-sensitive fluorescent probe. Phenoxazone dye that fluoresces intensely in variety of colours. Selective fluorescent stain for intracellular lipid droplets.
Purity >98%
Description Photostable polarity-sensitive fluorescent probe
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Nile Red product vial image | Hello Bio

Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in water or methanol (1mg/ml)
Storage of solutions Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >98%
Chemical name 9-(diethylamino)-5H-benzo[a]phenoxazin-5-one
Molecular Weight 318.37
Chemical structure Nile Red  [7385-67-3] Chemical Structure
Molecular Formula C20H18N2O2
CAS Number 7385-67-3
PubChem identifier 65182
SMILES CCN(CC)C1=CC2=C(C=C1)N=C3C4=CC=CC=C4C(=O)C=C3O2

References for Nile Red

References are publications that support the biological activity of the product
  • Nile red as a polarity-sensitive fluorescent probe of hydrophobic protein surfaces.

    Sackett DL et al (1987) Anal Biochem 167(2) : 228-34.
  • Application of Nile red, a fluorescent hydrophobic probe, for the detection of neutral lipid deposits in tissue sections: comparison with oil red O.

    Fowler SD et al (1985) J Histochem Cytochem 33(8) : 833-6.
  • Nile red: a selective fluorescent stain for intracellular lipid droplets.

    Greenspan P et al (1985) J Cell Biol 100(3) : 965-73.

3 Item(s)

Publications
These publications cite the use of Nile Red purchased from Hello Bio:
  • Autofluorescence of Model Polyethylene Terephthalate Nanoplastics for Cell Interaction Studies

    Lionetto F et al (2022) Nanomaterials (Basel) 12(9)
    PubMedID: 35564269

1 Item