Technical documents: SDS CoA Datasheet

Product overview

Name MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2
Biological description

SARS-CoV Mpro FRET substrate. The MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 substrate sequence is derived from residues P4–P5' of the SARS-CoV Mpro N-terminal autoprocessing site (AVLQSGFRK). Enzymatic cleavage between Q4 and S5 of MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 releases the highly fluorescent 7-methoxycoumarin (Mca) group from the influence of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large fluorescence intensity increase. This peptide is suitable to measure protease activity of the Mpro & SARS-CoV Mpro COVID-19 viruses in addition to other viruses.

Alternative names SARS Main Protease FRET Substrate
Purity >95%

SARS-CoV Mpro FRET substrate suitable for measuring Mpro & SARS-Cov Mpro protease activity

Write Your Own Review
You're reviewing:MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2
Rate this item:

Solubility & Handling

Storage instructions -20°C
Solubility overview

Soluble in DMSO

Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use



More Info


More Info

Chemical Data

Purity >95%
Molecular Weight 1514.7
Molecular Formula C69H99N19O15
Sequence (one letter) Mca-AVLQSGFRK(Dnp)K-NH2
Sequence (three letter) Mca-Ala-Val-Leu-Gln-Ser-Gly-Phe-Arg-Lys(Dnp)-Lys-NH2
CAS Number 932391-88-3

References for MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2

References are publications that support the biological activity of the product
  • Structure of the main protease from a global infectious human coronavirus, HCoV-HKU1

    Zhao Q et al (2008) J Virol 82(17) : 8647-55
  • Design of wide-spectrum inhibitors targeting coronavirus main proteases

    Yang H et al (2005) PLoS Biol 3(10) : e324

2 Item(s)