JC-10 is a highly soluble fluorescent probe ideal for assessing mitochondrial membrane potential. Â In healthy cells with polarized mitochondria, JC-10 aggregates, emitting a strong orange fluorescence (Ex/Em: 540nm/590nm). Â However, in cells with depolarized mitochondria, a hallmark of apoptosis and other cellular stresses, JC-10 reverts to its monomeric form, resulting in a shift to green fluorescence (Ex/Em: 490nm/525nm). This reversible, ratiometric change in fluorescence emission provides a reliable indicator of mitochondrial health. Â JC-10's superior aqueous solubility to JC-1 makes it a convenient and robust tool for various applications, including fluorescence microscopy, flow cytometry, and high-throughput screening.
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Handling
JC-10 is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
