Cell-permeable, yellow fluorescent dye supplied as a free acid. Suitable for confocal microscopy and super resolution microscopy (SRM) including techniques such as dSTORM (both live and fixed cells). Can also be multiplexed with Janelia Fluor ® 635 SE for two color imaging.
Fig1. Excitation and emission spectra of Janelia Fluor ® 525, free acid.
Figure1. Excitation and emission spectra of Janelia Fluor® 525, free acid. Janelia Fluor® 525 is a yellow fluorescent dye suitable for confocal and super resolution microscopy. A 1 µM solution was prepared in PBS before excitation, emission and absorbance spectra were generated. For protocol see #Protocol 1 in application notes below.
Fig1. Excitation and emission spectra of Janelia Fluor ® 525, free acid.
Figure1. Excitation and emission spectra of Janelia Fluor® 525, free acid. Janelia Fluor® 525 is a yellow fluorescent dye suitable for confocal and super resolution microscopy. A 1 µM solution was prepared in PBS before excitation, emission and absorbance spectra were generated. For protocol see #Protocol 1 in application notes below.
Optical Data
Emission color
Yellow
Max excitation wavelength
525 nm
Max emission wavelength
549 nm
Quantum Yield (φ)
0.91
Extinction Coefficient (ε)
122,000 M-1cm-1
Cell permeable
Yes
Reactive Group
Free acid
Biological Data
Application notes
#Protocol 1: Measurement of excitation and emission spectra of Janelia Fluor ® 525, free acid
Janelia Fluor ® 525, free acid was prepared at 1µm in PBS.
Spectra were generated on a Tecan Infinite M200 PRO using the following parameters:
Excitation: Recording at 618nm while exciting between 280nm and 590nm
Emission: Exciting at 484nm while recording between 510nm and 800nm
Absorbance: Measured between 300 and 800nm
Solubility & Handling
Storage instructions
-20°C
Solubility overview
Soluble in DMSO
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
