Cell-permeable, yellow fluorescent dye with a free acid reactive group. Used for the synthesis of Janelia Fluor® HaloTag® and SNAP-tag® ligands. Suitable for confocal microscopy and super resolution microscopy (SRM) including techniques such as dSTORM (both live and fixed cells). Can also be multiplexed with Janelia Fluor ® 635 SE for two color imaging.
Fig1. Excitation and emission spectra of Janelia Fluor ® 525, free acid.
Figure1. Excitation and emission spectra of Janelia Fluor® 525, free acid. Janelia Fluor® 525 is a yellow fluorescent dye suitable for confocal and super resolution microscopy. A 1 µM solution was prepared in PBS before excitation, emission and absorbance spectra were generated. For protocol see #Protocol 1 in application notes below.
Fig1. Excitation and emission spectra of Janelia Fluor ® 525, free acid.
Figure1. Excitation and emission spectra of Janelia Fluor® 525, free acid. Janelia Fluor® 525 is a yellow fluorescent dye suitable for confocal and super resolution microscopy. A 1 µM solution was prepared in PBS before excitation, emission and absorbance spectra were generated. For protocol see #Protocol 1 in application notes below.
Optical Data
Emission color
Yellow
Max excitation wavelength
525 nm
Max emission wavelength
549 nm
Quantum Yield (φ)
0.91
Extinction Coefficient (ε)
122,000 M-1cm-1
Cell permeable
Yes
Reactive Group
Free acid
Biological Data
Application notes
#Protocol 1: Measurement of excitation and emission spectra of Janelia Fluor ® 525, free acid
Janelia Fluor ® 525, free acid was prepared at 1µm in PBS.
Spectra were generated on a Tecan Infinite M200 PRO using the following parameters:
Excitation: Recording at 618nm while exciting between 280nm and 590nm
Emission: Exciting at 484nm while recording between 510nm and 800nm
Absorbance: Measured between 300 and 800nm
Solubility & Handling
Storage instructions
-20°C
Solubility overview
Soluble in DMSO
Storage of solutions
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
Yellow dye for coupling to primary amine groups. Suitable for super resolution microscopy (e.g. dSTORM, STED), confocal microscopy, live cell imaging and flow cytometry.
