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Images Fig1. Excitation and emission spectra of Janelia Fluor ® 549, azide. Figure1. Excitation and emission spectra of Janelia Fluor © 549, azide. Janelia Fluor® 549 is a yellow fluorescent dye suitable for confocal and super resolution microscopy. A 1 µM solution was prepared in PBS before excitation, emission and absorbance spectra were generated. For protocol see #Protocol 1 in application notes below.
Fig1. Excitation and emission spectra of Janelia Fluor ® 549, azide. Figure1. Excitation and emission spectra of Janelia Fluor © 549, azide. Janelia Fluor® 549 is a yellow fluorescent dye suitable for confocal and super resolution microscopy. A 1 µM solution was prepared in PBS before excitation, emission and absorbance spectra were generated. For protocol see #Protocol 1 in application notes below.
Optical Data Reactivity Click chemistry
Max excitation wavelength 549 nm
Max emission wavelength 571 nm
Extinction Coefficient (ε) 101,000 M-1 cm-1
Biological Data Application notes #Protocol 1: Measurement of excitation and emission spectra of Janelia Fluor ® 549, azide
Janelia Fluor® 549, azide was prepared at 1µm in PBS.
Spectra were generated on a Tecan Infinite M200 PRO using the following parameters:
Excitation: Recording at 638nm while exciting between 280nm and 610nm
Emission: Exciting at 509nm while recording between 535nm and 800nm
Absorbance: Measured between 300 and 800nm
Solubility & Handling Storage instructions -20°C
Solubility overview Soluble in DMSO
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
Chemical Data Chemical name 3,6-Di-1-azetidinyl-9-[5-[[2-[2-[2-[2-azidoethoxy]ethoxy]ethoxy]ethyl]carbamoyl]-2-carboxyphenyl]xanthylium, inner salt
Chemical structure
Molecular Formula C35 H38 N6 O7
PubChem identifier 137919860
SMILES O=C(NCCOCCOCCOCCN=[N+]=[N-])C1=CC=C(C([O-])=O)C(C(C2=CC=C(N3CCC3)C=C2O4)=C(C=C/5)C4=CC5=[N+]6CCC\6)=C1
InChiKey XDSXVSGQYCACTI-UHFFFAOYSA-N
Licensing details Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus
References for Janelia Fluor® 549, Azide References are publications that support the biological activity of the product
A general method to improve fluorophores for live-cell and single-molecule microscopy. Grimm JB et al (2015) Nature methods 12 : 244-50, 3 p following 250
Yellow dye supplied as an azide for click chemistry. Suitable for dSTORM, STED, confocal microscopy, live cell imaging and flow cytometry.
