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Product information
| Immunogen | Purified chicken IgY |
| Purification notes | Immunogen affinity chromatography. |
| Formulation | 20% glycerol in PBS with 0.05% sodium azide and 1% recombinant albumin |
Tested applications
| Applications | ELISA, FACS and flow cytometry, ICC, live cell imaging, WB, IHC(IF) |
| IHC(IF) optimal concentration | 1:300 to 1:2,000 dilution (0.5 - 3.3µg/ml). Optimise dependent upon assay. A good starting point is 1:500 (2µg/ml). |
| ICC optimal concentration | 1:300 to 1:2,000 dilution (0.5 - 3.3µg/ml). Optimise dependent upon assay. A good starting point is 1:500 (2µg/ml). |
Optical Data
| Max excitation wavelength | 646 nm |
| Max emission wavelength | 664 nm |
| Closest laser lines | 640nm |
| Spectrally similar dyes | Alexa Fluor® 647, Atto 647, DyLight® 650, Cyanine 5 |
| Fluorescence spectra |  |
Storage & Handling
| Storage instructions | +4°C |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
FAQs
What mounting media do you recommend to use with this antibody?
We recommend using one of our high performance mounting medias, supplied as either hardset or aqeous with a range of counterstains:
Hardset:
Aqueous:
Goat Anti-Chicken IgY H&L Janelia Fluor® 646 secondary antibody. Part of the ValidAb™ range of highly validated, data-rich antibodies.
Western Blot Protocol (1 MB)