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Product information Immunogen Purified chicken IgY
Purification notes Immunogen affinity chromatography.
Formulation 20% glycerol in PBS with 0.05% sodium azide and 1% recombinant albumin
Tested applications Applications ELISA, FACS and flow cytometry, ICC, live cell imaging, WB, IHC(IF)
IHC(IF) optimal concentration 1:300 to 1:2,000 dilution (0.5 - 3.3µg/ml). Optimise dependent upon assay. A good starting point is 1:500 (2µg/ml).
ICC optimal concentration 1:300 to 1:2,000 dilution (0.5 - 3.3µg/ml). Optimise dependent upon assay. A good starting point is 1:500 (2µg/ml).
Optical Data Max excitation wavelength 646 nm
Max emission wavelength 664 nm
Closest laser lines 640nm
Spectrally similar dyes Alexa Fluor® 647, Atto 647, DyLight® 650, Cyanine 5
Fluorescence spectra
Storage & Handling Storage instructions +4°C
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Storage of solutions Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
FAQs What mounting media do you recommend to use with this antibody?
We recommend using one of our high performance mounting medias, supplied as either hardset or aqeous with a range of counterstains:
Hardset:
Aqueous:
Goat Anti-Chicken IgY H&L Janelia Fluor® 646 secondary antibody. Part of the ValidAb ™ range of highly validated, data-rich antibodies.
Western Blot Protocol (1 MB)