Goat Anti-Chicken IgY H&L (Janelia Fluor® 646) ValidAbTM

(HB18908)
Technical documents: Datasheet

Product overview

Name Goat Anti-Chicken IgY H&L (Janelia Fluor® 646) ValidAbTM
Host Goat
Clonality Polyclonal
Target Chicken IgY H&L
Conjugate Janelia Fluor® 646
Description

Goat Anti-Chicken IgY H&L Janelia Fluor® 646 secondary antibody. Part of the ValidAb™ range of highly validated, data-rich antibodies.

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Product information

Immunogen

Purified chicken IgY

Purification notes

Immunogen affinity chromatography. 

Concentration 1mg/ml
Formulation 20% glycerol in PBS with 0.05% sodium azide and 1% recombinant albumin

Tested applications

Applications ELISA, FACS and flow cytometry, ICC, live cell imaging, WB, IHC(IF)
IHC(IF) optimal concentration

1:300 to 1:2,000 dilution (0.5 - 3.3µg/ml). Optimise dependent upon assay. A good starting point is 1:500 (2µg/ml).

ICC optimal concentration

1:300 to 1:2,000 dilution (0.5 - 3.3µg/ml). Optimise dependent upon assay. A good starting point is 1:500 (2µg/ml).

Optical Data

Max excitation wavelength 646 nm
Max emission wavelength 664 nm
Emission color Red
Closest laser lines 640nm
Spectrally similar dyes Alexa Fluor® 647, Atto 647, DyLight® 650, Cyanine 5
Fluorescence spectra Fluorescence Spectra

Storage & Handling

Storage instructions

+4°C

Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Storage of solutions Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use