Figure 1. ATP induced Ca2+ release in CHO-K1 cells measured using Fluo-4 AM
ATP induces Ca2+ release in a dose dependent manner in CHO-K1 cells which can be measured using Fluo-4 AM. Method: CHO-K1 cells were cultured in a 96-well plate following established protocols and transferred to a FlexStation. Following recording of a 30 second baseline, ATP was added to cells at either 0.1, 1.7, 50 or 10µM concentrations and the resulting increase in fluorescence at 525nm measured. For more details please see our Fluo-4 AM Protocol.
Figure 2. ΔF following ATP application to CHO-K1 cells.
Example image showing the increase in fluorescence of Fluo-4 AM in CHO-K1 cells following application of ATP. For more details please see our Fluo-4 AM Protocol.
Figure 1. ATP induced Ca2+ release in CHO-K1 cells measured using Fluo-4 AM
ATP induces Ca2+ release in a dose dependent manner in CHO-K1 cells which can be measured using Fluo-4 AM. Method: CHO-K1 cells were cultured in a 96-well plate following established protocols and transferred to a FlexStation. Following recording of a 30 second baseline, ATP was added to cells at either 0.1, 1.7, 50 or 10µM concentrations and the resulting increase in fluorescence at 525nm measured. For more details please see our Fluo-4 AM Protocol.
Figure 2. ΔF following ATP application to CHO-K1 cells.
Example image showing the increase in fluorescence of Fluo-4 AM in CHO-K1 cells following application of ATP. For more details please see our Fluo-4 AM Protocol.
This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
Can I fix my cells after loading with Fluo-4 AM dye for Ca2+ detection?
No. Fluo-4 AM isn't covalently bound to any cellular components and fixation damages the membrane meaning that the dye would leak out of the cell.
How can I prevent Fluo-4 leaking out of cells?
Fluo-4 AM generally has very good cell retention with minimal leakage. If excessive leakage is observed try either reducing the incubation temperature or adding probenecid to the loading solution.
Can unused dye be frozen and reused?
Once dye has been dissolved in DMSO then it is possible to aliquot and freeze at -20°C. Try to avoid more than 2 freeze/thaw cycles.
Fluo-4 AM Protocol (222.8 KB) 
