Product overview
| Name | Cell Counting Kit-8 (CCK-8) |
| Biological description | Cell Counting Kit-8 (CCK-8) is a ready to use solution for cell viability assays and cell proliferation assays. The kit uses WST-8 tetrazolium salt which is reduced by dehydrogenases in living cells to give a brightly coloured dye. The dye generated is directly proportional to the number of live cells enabling colorimetric quantitation of viable cell number. Key features:
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| Description | Ready to use solution for colorimetric quantitation of viable cell number. |
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Cell Counting Assay
HEK 293T cells at 0 to 300,000 cells in 100 µL were plated in a 96 well plate. 10 µL of CCK-8 were added to each well and the plate was incubated at 37°C and absorbance was measured at 450 nm at 1, 2, 3 and 4 hours using a plate reader.
Cytotoxicity test of Staurosporine on HEK293T cells
HEK293T cells were plated at 10,000 cells per well in 100 µL in a 96 well plate and incubated overnight. Cells were treated with different concentrations of Staurosporine (HB0590) for 4 hours before adding 10 µL of CCK-8. Absorbance at 450 nm was measured after 3 hours at 37°C and cell viability was calculated as a percentage of untreated cells.
Cytotoxicity test of SDS on HEK293T cells
HEK293T cells were plated at 10,000 cells per well in 100 µL in a 96 well plate and cultured overnight. Cells were treated with different concentrations of SDS for 2 hours before adding 10 µL of CCK-8. Absorbance at 450 nm was measured after 3 hours at 37°C and cell viability was calculated as a percentage of untreated cells.
Cell Counting Assay
HEK 293T cells at 0 to 300,000 cells in 100 µL were plated in a 96 well plate. 10 µL of CCK-8 were added to each well and the plate was incubated at 37°C and absorbance was measured at 450 nm at 1, 2, 3 and 4 hours using a plate reader.
Cytotoxicity test of Staurosporine on HEK293T cells
HEK293T cells were plated at 10,000 cells per well in 100 µL in a 96 well plate and incubated overnight. Cells were treated with different concentrations of Staurosporine (HB0590) for 4 hours before adding 10 µL of CCK-8. Absorbance at 450 nm was measured after 3 hours at 37°C and cell viability was calculated as a percentage of untreated cells.
Cytotoxicity test of SDS on HEK293T cells
HEK293T cells were plated at 10,000 cells per well in 100 µL in a 96 well plate and cultured overnight. Cells were treated with different concentrations of SDS for 2 hours before adding 10 µL of CCK-8. Absorbance at 450 nm was measured after 3 hours at 37°C and cell viability was calculated as a percentage of untreated cells.
Biological Data
| Application notes | Cell number determination
Cell proliferation and cytotoxicity assays
Notes
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Solubility & Handling
| Storage instructions | +4°C |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
References for Cell Counting Kit-8 (CCK-8)
References are publications that support the biological activity of the product
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Cell Viability Assay with 3D Prostate Tumor Spheroids.
Oner E et al (2023) Methods in molecular biology (Clifton, N.J.) 2645 : 263-275 -
Comparative Evaluation of Corneal Storage Medias Used as Tooth Avulsion Medias in Maintaining the Viability of Periodontal Ligament Cells Using the Cell Counting Kit-8 Assay.
James N et al (2022) Clinical, cosmetic and investigational dentistry 14 : 87-94 -
Comparison of Cytotoxicity Evaluation of Anticancer Drugs between Real-Time Cell Analysis and CCK-8 Method.
Cai L et al (2019) ACS omega 4 : 12036-12042
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