Product overview

Name Cell Counting Kit-8 (CCK-8)
Biological description

Cell Counting Kit-8 (CCK-8) is a ready to use solution for cell viability assays and cell proliferation assays. The kit uses WST-8 tetrazolium salt which is reduced by dehydrogenases in living cells to give a brightly coloured dye. The dye generated is directly proportional to the number of live cells enabling colorimetric quantitation of viable cell number. 

Key features:

  • Ready to use solution
  • Results after 1-4 hour incubation
  • The Cell Counting Kit-8 assay is more sensitive than other tetrazolium salt-based assays such as XTT, MTS and MTT.
  • Low cytotoxicity and high stability make this kit suitable for long incubation time (24-48 hours)
Description

Ready to use solution for colorimetric quantitation of viable cell number.

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Images

Cytotoxicity test of Staurosporine on HEK293T cells

HEK293T cells were plated at 10,000 cells per well in 100 µL in a 96 well plate and incubated overnight. Cells were treated with different concentrations of Staurosporine (HB0590) for 4 hours before adding 10 µL of CCK-8. Absorbance at 450 nm was measured after 3 hours at 37°C and cell viability was calculated as a percentage of untreated cells.

Cell Counting Assay

HEK 293T cells at 0 to 300,000 cells in 100 µL were plated in a 96 well plate. 10 µL of CCK-8 were added to each well and the plate was incubated at 37°C for 1 hour and absorbance was measured at 450 nm using a plate reader.

Cytotoxicity test of SDS on HEK293T cells

HEK293T cells were plated at 10,000 cells per well in 100 µL in a 96 well plate and cultured overnight. Cells were treated with different concentrations of SDS for 2 hours before adding 10 µL of CCK-8. Absorbance at 450 nm was measured after 3 hours at 37°C and cell viability was calculated as a percentage of untreated cells.
Cell Counting Kit-8 (CCK-8) (HB9337) product image

Biological Data

Application notes

Please follow this link to a full Cell Counting Kit-8 protocol.

Solubility & Handling

Storage instructions +4°C
Storage of solutions Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Technical guides

References for Cell Counting Kit-8 (CCK-8)

References are publications that support the biological activity of the product
  • Cell Viability Assay with 3D Prostate Tumor Spheroids.

    Oner E et al (2023) Methods in molecular biology (Clifton, N.J.) 2645 : 263-275
  • Comparative Evaluation of Corneal Storage Medias Used as Tooth Avulsion Medias in Maintaining the Viability of Periodontal Ligament Cells Using the Cell Counting Kit-8 Assay.

    James N et al (2022) Clinical, cosmetic and investigational dentistry 14 : 87-94
  • Comparison of Cytotoxicity Evaluation of Anticancer Drugs between Real-Time Cell Analysis and CCK-8 Method.

    Cai L et al (2019) ACS omega 4 : 12036-12042

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