Suitable as substrate for alkaline phosphatase detection in applications such as IHC & immunoblotting (e.g. Dot blot). Produces an insoluble, blue- purple end product following reaction with alkaline phosphatase (AP).
Contents
Solution of 0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2
Description
Alkaline phosphatase detection substrate. Ready to use solution.
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
Understanding purity and quality - a guide for life scientists
