NBT/BCIP Solution (Ready to Use)

Ready to use formulation:

Overview

Suitable as substrate for alkaline phosphatase detection in applications such as IHC & immunoblotting (e.g. Dot blot). Produces an insoluble, blue- purple end product following reaction with alkaline phosphatase (AP).

Contents

Solution of 0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2

Alkaline phosphatase detection substrate. Ready to use solution.