Ready to use cell lysis and protein solubilization buffer for adherent and suspension cultured mammalian cells. Extracts cytoplasmic, nuclear and membrane proteins. The gentle detergent in this buffer allows solubilization of protein complexes that retain enzymatic activity but may therefore result in less complete extractions when compared to other buffers (e.g RIPA buffers) that use more stringent detergents.
Description
Ready to use cell lysis and protein solubilization buffer