Cell-permeable, yellow fluorescent dye supplied as free acid. Used for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands for use in live cell imaging experiments (Grimm et al 2017) . Also suitable for flow cytometry. Janelia Fluor® 549 is 2 x brighter than TMR and Cy3 in vitro and live-cell experiments.
Fig1. Excitation and emission spectra of Janelia Fluor ® 549, free acid.
Figure1. Excitation and emission spectra of Janelia Fluor® 549, free acid. Janelia Fluor® 549 is a yellow fluorescent dye suitable for confocal and super resolution microscopy. A 1 µM solution was prepared in PBS before excitation, emission and absorbance spectra were generated. For protocol see #Protocol 1 in application notes below.
Fig1. Excitation and emission spectra of Janelia Fluor ® 549, free acid.
Figure1. Excitation and emission spectra of Janelia Fluor® 549, free acid. Janelia Fluor® 549 is a yellow fluorescent dye suitable for confocal and super resolution microscopy. A 1 µM solution was prepared in PBS before excitation, emission and absorbance spectra were generated. For protocol see #Protocol 1 in application notes below.
Optical Data
Emission color
Yellow
Max excitation wavelength
549 nm
Max emission wavelength
571 nm
Quantum Yield (φ)
0.88
Extinction Coefficient (ε)
101,000 M-1cm-1
Cell permeable
Yes
Reactive Group
Free acid
Biological Data
Application notes
#Protocol 1: Measurement of excitation and emission spectra of Janelia Fluor ® 549, free acid
Janelia Fluor® 549, free acid was prepared at 1µm in PBS.
Spectra were generated on a Tecan Infinite M200 PRO using the following parameters:
Excitation: Recording at 638nm while exciting between 280nm and 610nm
Emission: Exciting at 509nm while recording between 535nm and 800nm
Absorbance: Measured between 300 and 800nm
Solubility & Handling
Storage instructions
-20°C
Solubility overview
Soluble in DMSO (100 mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
![Janelia Fluor® 549, free acid [2245946-45-4] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb8745_1.png "Janelia Fluor® 549, free acid [2245946-45-4]")
