Cell-permeable, yellow fluorescent dye with a free acid reactive group. Used for the synthesis of Janelia Fluor® HaloTag® and SNAP-Tag® ligands for use in live cell imaging experiments (Grimm et al 2017) . Also suitable for flow cytometry. Janelia Fluor® 549 is 2 x brighter than TMR and Cy3 in vitro and live-cell experiments.
Fig1. Excitation and emission spectra of Janelia Fluor ® 549, free acid.
Figure1. Excitation and emission spectra of Janelia Fluor® 549, free acid. Janelia Fluor® 549 is a yellow fluorescent dye suitable for confocal and super resolution microscopy. A 1 µM solution was prepared in PBS before excitation, emission and absorbance spectra were generated. For protocol see #Protocol 1 in application notes below.
Fig1. Excitation and emission spectra of Janelia Fluor ® 549, free acid.
Figure1. Excitation and emission spectra of Janelia Fluor® 549, free acid. Janelia Fluor® 549 is a yellow fluorescent dye suitable for confocal and super resolution microscopy. A 1 µM solution was prepared in PBS before excitation, emission and absorbance spectra were generated. For protocol see #Protocol 1 in application notes below.
Optical Data
Emission color
Yellow
Max excitation wavelength
549 nm
Max emission wavelength
571 nm
Quantum Yield (φ)
0.88
Extinction Coefficient (ε)
101,000 M-1cm-1
Cell permeable
Yes
Reactive Group
Free acid
Biological Data
Application notes
#Protocol 1: Measurement of excitation and emission spectra of Janelia Fluor ® 549, free acid
Janelia Fluor® 549, free acid was prepared at 1µm in PBS.
Spectra were generated on a Tecan Infinite M200 PRO using the following parameters:
Excitation: Recording at 638nm while exciting between 280nm and 610nm
Emission: Exciting at 509nm while recording between 535nm and 800nm
Absorbance: Measured between 300 and 800nm
Solubility & Handling
Storage instructions
-20°C
Solubility overview
Soluble in DMSO (100 mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
Yellow dye for coupling to primary amine groups. Suitable for super resolution microscopy (e.g. dSTORM, STED), confocal microscopy, live cell imaging and flow cytometry.
![Janelia Fluor® 549, free acid [2245946-45-4] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb8745_1.png "Janelia Fluor® 549, free acid [2245946-45-4]")
