Cation dye. Used in Gram staining to distinguish between gram positive and negative bacteria. Interacts with aqueous form of Kl-I2 during gram staining producing a chemical precipitate. Displays colour changes at different pHs (pH0.0- yellow, pH 2.0- blue/violet). Also used to measure Cytostatic/cytotoxic effects on tumour cell lines.
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
Chemical mechanism of the Gram stain and synthesis of a new electron-opaque marker for electron microscopy which replaces the iodine mordant of the stain.
Davies JA et al (1983) J Bacteriol 156(2) : 837-45.