Cation dye. Used in Gram staining to distinguish between gram positive and negative bacteria. Interacts with aqueous form of Kl-I2 during gram staining producing a chemical precipitate. Displays colour changes at different pHs (pH0.0- yellow, pH 2.0- blue/violet). Also used to measure Cytostatic/cytotoxic effects on tumour cell lines.
Chemical mechanism of the Gram stain and synthesis of a new electron-opaque marker for electron microscopy which replaces the iodine mordant of the stain.
Davies JA et al (1983) J Bacteriol 156(2) : 837-45.