Product overview

Name Crystal Violet
Alternative names Basic Violet 3, Gentian Violet, Hexamethylpararosaniline chloride, Methyl Violet 10B
Biological description Cation dye. Used in Gram staining to distinguish between gram positive and negative bacteria. Interacts with aqueous form of Kl-I2 during gram staining producing a chemical precipitate. Displays colour changes at different pHs (pH0.0- yellow, pH 2.0- blue/violet). Also used to measure Cytostatic/cytotoxic effects on tumour cell lines.
Description Cation dye used in Gram staining
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Images

Crystal Violet product vial image | Hello Bio

Solubility & Handling

Storage instructions Room temperature
Solubility overview Soluble in water (50mg/ml, at 27°C) or chloroform
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

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Dilution

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Chemical Data

Chemical name Gentian Violet; Hexamethylpararosaniline chloride
Molecular Weight 407.99
Chemical structure Crystal Violet  [548-62-9] Chemical Structure
Molecular Formula C25H30N3Cl
CAS Number 548-62-9
PubChem identifier 11057
SMILES [Cl-].CN(C)C1=CC=C(C=C1)C(\C1=CC=C(C=C1)N(C)C)=C1/C=C\C(\C=C/1)=[N+](/C)C

References for Crystal Violet

References are publications that support the biological activity of the product
  • Crystal violet as an i-motif structure probe for reversible and label-free pH-driven electrochemical switch.

    Zhang XY et al (2014) Anal Biochem 455 : 55-9.
  • Statins potentiate cytostatic/cytotoxic activity of sorafenib but not sunitinib against tumor cell lines in vitro.

    Bil J et al (2010) Cancer Lett 288(1) : 57-67.
  • Chemical mechanism of the Gram stain and synthesis of a new electron-opaque marker for electron microscopy which replaces the iodine mordant of the stain.

    Davies JA et al (1983) J Bacteriol 156(2) : 837-45.

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