Product overview
| Name | Calcein-AM |
| Description | Non- fluorescent, cell permeable dye |
| Purity | >95% |
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Biological Data
| Biological description | Non- fluorescent and cell permeable dye. Hydrolysed into green fluorescent calcein by cytosolic esterases in living cells. Uses include monitoring chemotaxis, multidrug resistance and determining cell viability. |
Solubility & Handling
| Storage instructions | -20°C |
| Solubility overview | Soluble in DMSO (10mg/ml) or water |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
| Chemical name | CAL-AM |
| Molecular Weight | 994.86 |
| Chemical structure | ![Calcein-AM [148504-34-1] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0720.png "Calcein-AM [148504-34-1]") |
| Molecular Formula | C46H46N2O23 |
| CAS Number | 148504-34-1 |
| PubChem identifier | 390986 |
| SMILES | CC(=O)OCOC(=O)CN(CC1=C(C=CC2=C1OC3=C(C24C5=CC=CC=C5C(=O)O4)C=CC(=C3CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OC(=O)C)OC(=O)C)CC(=O)OCOC(=O)C |
| InChiKey | XKFSBWQWNMZWFA-UHFFFAOYSA-N |
References for Calcein-AM
References are publications that support the biological activity of the product
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Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging.
Bratosin D et al (2005) Cytometry A 66(1) : 78-84. -
Membrane topology and glycosylation of the human multidrug resistance-associated protein.
Bakos E et al (1996) J Biol Chem 271(21) : 12322-6. -
The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay.
De Gendt CM et al (1996) Clin Chim Acta 249(1-2) : 189-95.
Publications
These publications cite the use of Calcein-AM purchased from Hello Bio:
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Développement de nouveaux biosenseurs fluorescents pour l’étude dynamique de la signalisation purinergique.
Matthias Ollivier (2018) Sciences agricoles. Université Montpellier : Thesis
Understanding purity and quality - a guide for life scientists