Calcein-AM

(HB0720)
Technical documents: SDS Datasheet
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Calcein-AM product vial image | Hello Bio

Biological Data

Biological description Non- fluorescent and cell permeable dye. Hydrolysed into green fluorescent calcein by cytosolic esterases in living cells. Uses include monitoring chemotaxis, multidrug resistance and determining cell viability.

Solubility & Handling

Solubility overview Soluble in DMSO (10mg/ml) or water
Storage instructions -20°C
Storage of solutions Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >90%
Chemical name CAL-AM
Molecular Weight 994.86
Chemical structure Calcein-AM  [148504-34-1] Chemical Structure
Molecular Formula C46H46N2O23
CAS Number 148504-34-1
PubChem identifier 390986
SMILES CC(=O)OCOC(=O)CN(CC1=C(C=CC2=C1OC3=C(C24C5=CC=CC=C5C(=O)O4)C=CC(=C3CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OC(=O)C)OC(=O)C)CC(=O)OCOC(=O)C
InChiKey XKFSBWQWNMZWFA-UHFFFAOYSA-N

References for Calcein-AM

References are publications that support the biological activity of the product
  • Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging.

    Bratosin D et al (2005) Cytometry A 66(1) : 78-84.
  • Membrane topology and glycosylation of the human multidrug resistance-associated protein.

    Bakos E et al (1996) J Biol Chem 271(21) : 12322-6.
  • The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay.

    De Gendt CM et al (1996) Clin Chim Acta 249(1-2) : 189-95.

3 Item(s)

Publications
These publications cite the use of Calcein-AM purchased from Hello Bio:
  • Développement de nouveaux biosenseurs fluorescents pour l’étude dynamique de la signalisation purinergique.

    Matthias Ollivier (2018) Sciences agricoles. Université Montpellier : Thesis

1 Item