Calcein-AM

(HB0720)
Technical documents: SDS Datasheet
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Calcein-AM product vial image | Hello Bio

Biological Data

Biological description Non- fluorescent and cell permeable dye. Hydrolysed into green fluorescent calcein by cytosolic esterases in living cells. Uses include monitoring chemotaxis, multidrug resistance and determining cell viability.

Solubility & Handling

Storage instructions -20°C
Solubility overview Soluble in DMSO (10mg/ml) or water
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >90%
Chemical name CAL-AM
Molecular Weight 994.86
Chemical structure Calcein-AM  [148504-34-1] Chemical Structure
Molecular Formula C46H46N2O23
CAS Number 148504-34-1
PubChem identifier 390986
SMILES CC(=O)OCOC(=O)CN(CC1=C(C=CC2=C1OC3=C(C24C5=CC=CC=C5C(=O)O4)C=CC(=C3CN(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OC(=O)C)OC(=O)C)CC(=O)OCOC(=O)C
InChiKey XKFSBWQWNMZWFA-UHFFFAOYSA-N

References for Calcein-AM

References are publications that support the biological activity of the product
  • Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging.

    Bratosin D et al (2005) Cytometry A 66(1) : 78-84.
  • Membrane topology and glycosylation of the human multidrug resistance-associated protein.

    Bakos E et al (1996) J Biol Chem 271(21) : 12322-6.
  • The use of calcein acetomethylester (AM)-labelled polymorphonuclear cells in a polycarbonate filter chemotaxis assay.

    De Gendt CM et al (1996) Clin Chim Acta 249(1-2) : 189-95.

3 Item(s)

Publications
These publications cite the use of Calcein-AM purchased from Hello Bio:
  • Développement de nouveaux biosenseurs fluorescents pour l’étude dynamique de la signalisation purinergique.

    Matthias Ollivier (2018) Sciences agricoles. Université Montpellier : Thesis

1 Item