Figure1. Protein expression in rat brain P2 membrane and cytosol fractions.
Brilliant Blue R-250 is commonly used to stain proteins following SDS-PAGE. Brilliant Blue R-250 from Hello Bio specifically labels bands of protein obtained from rat brain P2 and cytosol fractions following SDS- PAGE. For protocol see #Protocol 1 in application notes below.
Brilliant Blue R-250 product vial image | Hello Bio
Figure1. Protein expression in rat brain P2 membrane and cytosol fractions.
Brilliant Blue R-250 is commonly used to stain proteins following SDS-PAGE. Brilliant Blue R-250 from Hello Bio specifically labels bands of protein obtained from rat brain P2 and cytosol fractions following SDS- PAGE. For protocol see #Protocol 1 in application notes below.
Brilliant Blue R-250 product vial image | Hello Bio
Biological Data
Application notes
#Protocol 1: Brilliant Blue R-250 staining of rat brain fractions.
P2 membrane and cytosol fractions were prepared from rat brains following established protocols (Molnar et al., 1993. Neuroscience 53:307-326).
SDS-PAGE was conducted following standard protocols (Laemmli., 1970. Nature 227:680-685) using a 10% acrylamide gel.
Staining and de-staining solutions were prepared as:
Staining:
Reagent
Concentration
Amount for 250ml
Brilliant Blue R-250 (HB0739)
0.025%
62.5mg
Methanol
40%
100ml
Acetic acid
7%
17.5ml
dH2O
53%
132.5ml
De-staining:Â
Reagent
Concentration
Amount for 250ml
Methanol
50%
125ml
Acetic acid
7%
17.5ml
dH2O
43%
107.5ml
The gel was incubated in staining solution for 20 minutes followed by incubation in destaining solution for 3 hours with multiple changes of solution.
Imaging was conducted using a Epson 4180 Photo scanner
Solubility & Handling
Storage instructions
Room temperature
Solubility overview
Water (10mM)
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
![Brilliant Blue R-250 [6104-59-2] Chemical Structure](https://hellobio.com/media/catalog/product//h/b/hb0739.png "Brilliant Blue R-250 [6104-59-2]")
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