Product overview

Name 1% NH4OH solution
Biological description

1% NH4OH solution which can be used as the solvent for Beta-Amyloid (1-42) and (1-40) peptides.

Please ensure that you are using the appropriate solubilization buffer/ methodology for reconstituting your Beta-amyloid peptide.

When using Beta-amyloid peptides (1-42) or (1-40), you should use 1.0% NH4OH as the solvent followed by buffer (for example 1X PBS).

  1. Add 1.0% NH4OH directly to the lyophilized peptide (~70-80 μl for 1mg of peptide). Do not store the peptide in 1.0% NH4OH.
  2. Immediately dilute your solution to a concentration of ~1mg/mL or less with 1X PBS or alternative buffer.
  3. Vortex gently to mix (less than 1 minute).

Note: This method may not completely remove pre-aggregates. Vortexing may encourage seeding and further aggregation of the peptide.

Alternative names 1% Ammonia solution
Description 1% NH4OH solution which can be used as the solvent for Beta-Amyloid (1-42) and (1-40) peptides
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Solubility & Handling

Storage instructions Room temperature
Storage of solutions Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

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Dilution

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Chemical Data

Chemical name Ammonium hydroxide
Molecular Weight 35
Molecular Formula NH5O
CAS Number 7664-41-7
Appearance colorless solution

References for 1% NH4OH solution

References are publications that support the biological activity of the product
  • Ammonium hydroxide treatment of Aβ produces an aggregate free solution suitable for biophysical and cell culture characterization.

    Ryan et al (2013) PeerJ 7;1 : e73

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