Product overview

Name 1% NH4OH solution
Description 1% NH4OH solution which can be used as the solvent for Beta-Amyloid (1-42) and (1-40) peptides
Biological description

1% NH4OH solution which can be used as the solvent for Beta-Amyloid (1-42) and (1-40) peptides.

Please ensure that you are using the appropriate solubilization buffer/ methodology for reconstituting your Beta-amyloid peptide.

When using Beta-amyloid peptides (1-42) or (1-40), you should use 1.0% NH4OH as the solvent followed by buffer (for example 1X PBS).

  1. Add 1.0% NH4OH directly to the lyophilized peptide (~70-80 μl for 1mg of peptide). Do not store the peptide in 1.0% NH4OH.
  2. Immediately dilute your solution to a concentration of ~1mg/mL or less with 1X PBS or alternative buffer.
  3. Vortex gently to mix (less than 1 minute).

Note: This method may not completely remove pre-aggregates. Vortexing may encourage seeding and further aggregation of the peptide.

Alternative names 1% Ammonia solution
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Solubility & Handling

Storage instructions Room temperature
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use

Calculators

Molarity

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Dilution

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Chemical Data

Concentration 1%
Appearance colorless solution

References for 1% NH4OH solution

References are publications that support the biological activity of the product
  • Ammonium hydroxide treatment of Aβ produces an aggregate free solution suitable for biophysical and cell culture characterization.

    Ryan et al (2013) PeerJ 7;1 : e73