Product overview
Name | Sucrose aCSF Instant Powder (packets) |
Biological description | Sucrose artificial cerebrospinal fluid (sucrose - aCSF) is a widely used buffer as a protective cutting solution when making acute ex-vivo brain slices for electrophysiology experiments. This kit contains 10 instant powder packets. Simply dissolve the contents of each packet in dH2O to a final volume of 1L, mix and bubble with carbogen to make 1L of sucrose aCSF at physiological pH. Â Key features:
Contains (in mM): Sucrose 65, NaCl 85, Glucose 10, NaHCO3 25, KCl 2.5, NaH2PO4 1.25, CaCl2 0.5, MgCl2 7 |
Description | Preformulated instant powder packets to make sucrose artificial cerebrospinal fluid (sucrose - aCSF) |
Solubility & Handling
Storage instructions | RT. Dissolve each pack in dH2O to 1L final volume. |
Handling | Dissolve the contents of each packet in dH2O to a final volume of 1000ml, mix well and bubble with carbogen (10-15 minutes) to make 1L of sucrose aCSF at physiological pH. Use immediately once opened. |
Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use |
Chemical Data
Kit contents | Preformulated packets. Each makes 1L of sucrose - aCSF. |
References for Sucrose aCSF Instant Powder (packets)
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Repeated whole-cell patch-clamp recording from CA1 pyramidal cells in rodent hippocampal slices followed by axon initial segment labeling.
Oliveira LS et al (2021) STAR protocols 2 : 100336 -
Acute brain slice methods for adult and aging animals: application of targeted patch clamp analysis and optogenetics.
Ting JT et al (2014) Methods in molecular biology (Clifton, N.J.) 1183 : 221-42 -
Reduced long-term potentiation in hippocampal slices prepared using sucrose-based artificial cerebrospinal fluid.
Kuenzi FM et al (2000) Journal of neuroscience methods 100 : 117-22