Puromycin dihydrochloride
(HB4041)
Product overview
| Name | Puromycin dihydrochloride |
| Purity | >98% |
| Description | Protein synthesis inhibitor. Cell culture selection antibiotic. |
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Biological Data
| Biological description | Aminonucleoside antibiotic which disrupts peptide transfer on ribosomes on both prokaryotic and eukaryotic ribosomes to cause premature chain termination during translation. It inhibits protein transport of into the mitochondria and also induces apoptosis and shows antineoplastic and antibacterial activity. It is widely used as a selection antibiotic in cell culture. Puromycin allows the selection of cells that contain the puromycin N-acetyl-transferase gene (PAC) resistance gene. |
Solubility & Handling
| Storage instructions | -20°C |
| Solubility overview | Soluble in DMSO (100 mM) and water (100 mM) |
| Handling | This compound is light sensitive; exposure to light may affect compound performance. We therefore recommend storing the solid material and any solutions in the dark and protecting from light. |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
| Purity | >98% |
| Chemical name | 3'-[α-Amino-p-methoxyhydrocinnamamido]-3'-deoxy-N,N-dimethyladenosine dihydrochloride |
| Molecular Weight | 544.43 |
| Molecular Formula | C22H29N7O5.2HCl |
| CAS Number | 58-58-2 |
| PubChem identifier | 443311 |
| SMILES | CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl |
| InChiKey | RXWNCPJZOCPEPQ-VJWOANPKSA-N |
| MDL number | MFCD00012691 |
| Appearance | White to off-white |
Technical guides
Understanding purity and quality - a guide for life scientistsReferences for Puromycin dihydrochloride
References are publications that support the biological activity of the product
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Complexes between nascent polypeptides and their molecular chaperones in the cytosol of mammalian cells.
Eggers et al (1997) Mol Biol Cell 8(8) : 1559-73 -
Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction.
Price and Verner (1993) Biochim Biophys Acta. 1150(1) : 89-97 -
Dipeptidyl arylamidase II of the pituitary. Properties of lysylalanyl-beta-naphthylamide hydrolysis: inhibition by cations, distribution in tissues, and subcellular localization.
McDonald et al (1968) J Biol Chem 243(8) : 2028-37
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