Protease Inhibitor Cocktail powder (General Use)

Overview

This protease inhibitor cocktail contains a mixture of protease inhibitors with a broad range for proteases and esterases. It has been optimized for the inhibition of a broad range of proteases and esterases. One vial makes 1ml of a 100x stock solution and is sufficient to inhibit proteases in 100ml of cell lysate.

Protease inhibitor cocktails protect proteins from degradation by endogenous proteases released during protein extraction and purification.

Components and action

The following components included in this cocktail target: serine proteases, cysteine proteases, metalloproteases.

• AEBSF hydrochloride serine protease inhibitor (500µM)
• Aprotinin (bovine, recombinant, Nicotiana sp.,): serine protease inhibitor (150nM)
• E-64: cysteine protease inhibitor (1µM)
• Leupeptin hemisulfate: serine/cysteine protease inhibitor (1µM)
• EDTA disodium salt: metalloprotease inhibitor (500µM)

Formulation & usage recommendation

The cocktail is supplied as a white lyophilized solid. Reconstitute each vial with 1ml of water to obtain a 100X concentrate stock solution.

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.