Product overview

Name Indo-1 AM
Description Calcium sensitive fluorescent dye
Alternative names Indo-1/AM; Indo-1 acetoxymethylester
Biological description Cell permeable calcium sensitive flouresecent dye. Binds to Ca2+ (Kd = 0.25 µM). Ratiometric and excited by UV light. Hydrolysed into Indo-1 by cytoplasmic esterases. May be used for monitoring Ca<2+> levels. Inhibits renal proximal tubule cell volume regulation.
Purity >95%
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Solubility & Handling

Storage instructions -20°C
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.



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Chemical Data

Chemical name 1-[2-Amino-5-(6-carboxyindol-2-yl)phenoxy]-2-(2\'-amino-5\'-methylphenoxy)ethane-N,N,N\',N\'-tetraacetic acid, pentaacetoxymethylester
Molecular Weight 1009.9
Chemical structure Indo-1 AM  [112926-02-0] Chemical Structure
Molecular Formula C47H51N3O22
CAS Number 112926-02-0
SMILES CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc(ccc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c3[nH]c4cc(ccc4c3)C(=O)OCOC(C)=O

References for Indo-1 AM

References are publications that support the biological activity of the product
  • The source of epifluorescence in isolated perfused heart loaded with fura 2-AM or indo 1-AM.

    Shinozaki T et al (1993) Heart Vessels 8(2) : 79-84.
  • The fluorescent calcium indicator indo-1/AM inhibits renal proximal tubule cell volume regulation.

    Kanli H et al (1992) Ann Clin Lab Sci 22(4) : 236-44.
  • Calcium mobilization in human platelets using indo-1 and flow cytometry.

    Jennings LK et al (1989) Blood 74(8) : 2674-80.
  • A new generation of Ca2+ indicators with greatly improved fluorescence properties.

    Grynkiewicz G et al (1985) J Biol Chem 260(6) : 3440-50.