DCFDA / H2DCFDA is a cell permeable fluorescent probe that is redox sensitive and used to measure the concentration of reactive oxygen species (ROS) within a population of cells. DCFDA / H2DCFDA diffuses into cells where it is hydrolysed by intracellular esterases into a non-fluorescent and non-cell permeable intermediate. Upon reaction with ROS this forms the fluorescent compound 2’,7’ –dichlorofluorescein (DCF) which is excited at 485nm and emits at 535nm. Pyocyanin is included within this kit as a positive control. Pyocyanin promotes the formation of ROS through inactivation of catalase and depleting reduced glutathione.
This kit contains:
DCFDA / H2DCFDA assay reagent
DMSO
Lyophilised Pyocyanin
10x assay buffer
Description
Kit for measurement of reactive oxygen species (ROS) within cells.
Figure 1. Concentration response of pyocyanin in HEK293T cells using HB7375 DCFDA / H2DCFDA - Cellular ROS Assay Kit
Figure 1. Concentration response of pyocyanin in HEK293T cells using HB7375 DCFDA / H2DCFDA - Cellular ROS Assay Kit
Optical Data
Emission color
Green
Max excitation wavelength
485nm
Max emission wavelength
535nm
Closest laser lines
488 nm
Cell permeable
Yes
Biological Data
Application notes
Please follow this link to a full DCFDA / H2DCFDA - Cellular ROS Assay Kit protocol
Solubility & Handling
Storage instructions
-20°C
Storage of solutions
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use
![H2DCFDA [4091-99-0] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb8322_1.png "H2DCFDA [4091-99-0]")
DCFDA / H2DCFDA - Cellular ROS Assay Kit Protocol
