CA200623 CellAura fluorescent adenosine agonist [NECA]
(HB7813)
Product overview
| Name | CA200623 CellAura fluorescent adenosine agonist [NECA] |
| Description | Fluorescent adenosine receptor agonist |
| Biological description | Fluorescent adenosine receptor agonist (pEC50 values are 8.57, 8.47, 6.76 and 5.69 for A3, A1, A2A and A2B respectively). Also inhibits forskolin-stimulated cAMP accumulation (pIC50 = 8.57) and [3H]-inositol phosphate accumulation (pEC50 = 7.34). A fluorescent adenosine receptor ligand derived from NECA, non-selective adenosine agonist. |
| Alternative names | Fluorescent Adenosine receptor Agonist (A-633-AG), A-633-AG, ABEA-X-BY630 |
| Purity | >97% |
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Images
![CA200623 CellAura fluorescent adenosine agonist [NECA]: Scientist Approved](https://hellobio.com/media/catalog/product/h/e/hello_bio_scientist_approved_1_5.jpg)
Biological Data
| Application notes | For ligand binding; fluorescence imaging; high content analysis; kinetic analysis; cell sorting at adenosine A1 / A2A / A3 receptors use solutions up to 100 nM. |
| Pharmacological validation | For full experimental data see: J. Med. Chem. 2007, 50, 782-793; FASEB J. 2008, 22, 850-860 |
Solubility & Handling
| Storage instructions | -20°C (protect from light) |
| Solubility overview | Soluble in DMSO |
| Handling | After thawing individual aliquots for use, we recommend briefly sonicating the sample to ensure it is fully dissolved and the solution is homogeneous. We do not recommend using the product after subjecting it to repetitive freeze-thaw cycles. |
| Shipping conditions | The product, supplied in a dry form, is stable at ambient temperature for periods of up to a few days and does not require shipping on ice/dry ice. |
| Important | This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use. |
Chemical Data
| Molecular Weight | 925 |
| Source | Synthetic |
| Appearance | Purple solid |
| Formulation | Lyophilized film |
| Excitation | 638 nm |
| Emission | 657 nm |
References for CA200623 CellAura fluorescent adenosine agonist [NECA]
References are publications that support the biological activity of the product
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Probe dependence of allosteric enhancers on the binding affinity of adenosine A1 -receptor agonists at rat and human A1 -receptors measured using NanoBRET.
Cooper SL et al (2019) Br J Pharmacol. : doi: 10.1111/bph.14575. [Epub ah
Publications
These publications cite the use of CA200623 CellAura fluorescent adenosine agonist [NECA] purchased from Hello Bio:
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NanoBRET ligand binding at a GPCR under endogenous promotion facilitated by CRISPR/Cas9 genome editing
White et al (2019) Cell Signal. 54 : 27-34PubMedID: 30471466 -
Metabotropic purinergic receptors in lipid membrane microdomains.
D' Ambrosi N et al. (2013) Curr Med Chem 20(1) : 56-63.PubMedID: 23151003 -
Adenosine receptors and membrane microdomains.
Lasley RD (2011) Biochim Biophys Acta 1808(5) : 1284-9.PubMedID: 20888790 -
Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A1 receptor ligands.
Baker JG et al. (2010) Br J Pharmacol 159(4) : 772-86.PubMedID: 20105183 -
Developments in fluorescent probes for receptor research.
Leopoldo M et al. (2009) Drug Discov Today 14(13-14) : 706-12.PubMedID: 19573791
Technical guides
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