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CA200623 CellAura fluorescent adenosine agonist [NECA]

(HB7813)
Publications (8)
Technical documents: SDS CoA Datasheet
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Product overview

Name CA200623 CellAura fluorescent adenosine agonist [NECA]
Biological description Fluorescent adenosine receptor agonist (pEC50 values are 8.57, 8.47, 6.76 and 5.69 for A3, A1, A2A and A2B respectively). Also inhibits forskolin-stimulated cAMP accumulation (pIC50 = 8.57) and [3H]-inositol phosphate accumulation (pEC50 = 7.34). A fluorescent adenosine receptor ligand derived from NECA, non-selective adenosine agonist.
Alternative names Fluorescent Adenosine receptor Agonist (A-633-AG), A-633-AG, ABEA-X-BY630
Purity >97%
Description Fluorescent adenosine receptor agonist
Biological Data Solubility & Handling Chemical Data Related Areas Calculators Technical guides (2) FAQs (1) Reviews Publications (8) References (1) Frequently bought together
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Images

Figure 1. A3-SPAP cells assayed with HB7813
Figure 1. A3-SPAP cells assayed with HB7813
Figure 2. A2A-SPAP cells assayed with HB7813
Figure 2. A2A-SPAP cells assayed with HB7813
Figure 3. A1-SPAP cells assayed with HB7813
Figure 3. A1-SPAP cells assayed with HB7813
Fluorescence imaging with HB7813

Fluorescence imaging with HB7813

HB7813(30 nM) binding to live CHO cells expressing adenosine A3 receptors. Binding blocked by unlabelled competitor XAC (10 µM). Nuclei counter-stained with Hoechst.
CA200623 CellAura fluorescent adenosine agonist [NECA]: Scientist Approved
CA200623 CellAura fluorescent adenosine agonist [NECA]: Scientist Approved
CellAura fluorescent adenosine agonist [NECA] product vial image | Hello Bio
CellAura fluorescent adenosine agonist [NECA] product vial image | Hello Bio

Figure 1. A3-SPAP cells assayed with HB7813

Figure 1. A3-SPAP cells assayed with HB7813

Figure 2. A2A-SPAP cells assayed with HB7813

Figure 2. A2A-SPAP cells assayed with HB7813

Figure 3. A1-SPAP cells assayed with HB7813

Figure 3. A1-SPAP cells assayed with HB7813
Fluorescence imaging with HB7813

Fluorescence imaging with HB7813

HB7813(30 nM) binding to live CHO cells expressing adenosine A3 receptors. Binding blocked by unlabelled competitor XAC (10 µM). Nuclei counter-stained with Hoechst.

CA200623 CellAura fluorescent adenosine agonist [NECA]: Scientist Approved

CA200623 CellAura fluorescent adenosine agonist [NECA]: Scientist Approved

CellAura fluorescent adenosine agonist [NECA] product vial image | Hello Bio

CellAura fluorescent adenosine agonist [NECA] product vial image | Hello Bio

Biological Data

Application notes For ligand binding; fluorescence imaging; high content analysis; kinetic analysis; cell sorting at adenosine A1 / A2A / A3 receptors use solutions up to 100 nM.
Pharmacological validation For full experimental data see: J. Med. Chem. 2007, 50, 782-793; FASEB J. 2008, 22, 850-860

Solubility & Handling

Storage instructions -20°C (protect from light)
Solubility overview Soluble in DMSO
Storage of solutions Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Handling After thawing individual aliquots for use, we recommend briefly sonicating the sample to ensure it is fully dissolved and the solution is homogeneous. We do not recommend using the product after subjecting it to repetitive freeze-thaw cycles.
Shipping Conditions Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.

Calculators

Molarity

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Dilution

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Chemical Data

Purity >97%
Molecular Weight 925
Source Synthetic
Appearance Purple solid
Formulation Lyophilized film
Excitation 638 nm
Emission 657 nm

References for CA200623 CellAura fluorescent adenosine agonist [NECA]

References are publications that support the biological activity of the product

  • Probe dependence of allosteric enhancers on the binding affinity of adenosine A1 -receptor agonists at rat and human A1 -receptors measured using NanoBRET.

    Cooper SL et al (2019) Br J Pharmacol. : doi: 10.1111/bph.14575. [Epub ah
    Read more (PubMedID: 30644086)

1 Item

Publications
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These publications cite the use of CA200623 CellAura fluorescent adenosine agonist [NECA] purchased from Hello Bio:

  • Novel curcumin analog (cis-trans curcumin) as ligand to adenosine receptors A2A and A2B: potential for therapeutics

    Hamilton LJ et al (2021) Pharmacol Res 165 : 105410
    PubMedID: 33401004

  • NanoBRET ligand binding at a GPCR under endogenous promotion facilitated by CRISPR/Cas9 genome editing

    White et al (2019) Cell Signal. 54 : 27-34
    PubMedID: 30471466

  • Metabotropic purinergic receptors in lipid membrane microdomains.

    D' Ambrosi N et al. (2013) Curr Med Chem 20(1) : 56-63.
    PubMedID: 23151003

  • Adenosine receptors and membrane microdomains.

    Lasley RD (2011) Biochim Biophys Acta 1808(5) : 1284-9.
    PubMedID: 20888790

  • Influence of fluorophore and linker composition on the pharmacology of fluorescent adenosine A1 receptor ligands.

    Baker JG et al. (2010) Br J Pharmacol 159(4) : 772-86.
    PubMedID: 20105183

Items 1 to 5 of 8 total

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50μg
$275
Fluorescent adenosine receptor agonist
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