HB7813(30 nM) binding to live CHO cells expressing adenosine A3 receptors. Binding blocked by unlabelled competitor XAC (10 µM). Nuclei counter-stained with Hoechst.
HB7813(30 nM) binding to live CHO cells expressing adenosine A3 receptors. Binding blocked by unlabelled competitor XAC (10 µM). Nuclei counter-stained with Hoechst.
For ligand binding; fluorescence imaging; high content analysis; kinetic analysis; cell sorting at adenosine A1 / A2A / A3 receptors use solutions up to 100 nM.
Pharmacological validation
For full experimental data see: J. Med. Chem. 2007, 50, 782-793; FASEB J. 2008, 22, 850-860
Solubility & Handling
Storage instructions
-20°C (protect from light)
Solubility overview
Soluble in DMSO
Handling
After thawing individual aliquots for use, we recommend briefly sonicating the sample to ensure it is fully dissolved and the solution is homogeneous. We do not recommend using the product after subjecting it to repetitive freeze-thaw cycles.
Shipping conditions
The product, supplied in a dry form, is stable at ambient temperature for periods of up to a few days and does not require shipping on ice/dry ice.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References for CA200623 CellAura fluorescent adenosine agonist [NECA]
References are publications that support the biological activity of the product
Probe dependence of allosteric enhancers on the binding affinity of adenosine A1 -receptor agonists at rat and human A1 -receptors measured using NanoBRET.
Cooper SL et al (2019) Br J Pharmacol. : doi: 10.1111/bph.14575. [Epub ah
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