Selective M3 muscarinic receptor antagonist. Blocks [3H]-scopolamine binding to muscle cells expressing M3 and M2 (IC50 values are 0.4 nM and 15 µM respectively).
Solubility & Handling
Solubility overview
Soluble in DMSO (25mM)
Storage instructions
Room temperature
Storage of solutions
Prepare and use solutions on the same day if possible. Store solutions at -20°C for up to one month if storage is required. Equilibrate to RT and ensure the solution is precipitate free before use.
Shipping Conditions
Stable for ambient temperature shipping. Follow storage instructions on receipt.
Important
This product is for RESEARCH USE ONLY and is not intended for therapeutic or diagnostic use. Not for human or veterinary use.
References are publications that support the biological activity of the product
The use of a modified [3H]4-DAMP radioligand binding assay with increased selectivity for muscarinic M3 receptor shows that cortical CHRM3 levels are not altered in mood disorders.
Jeon WJ et al (2013) Prog Neuropsychopharmacol Biol Psychiatry 47 : 7-12.
Differential coupling of muscarinic m2 and m3 receptors to adenylyl cyclases V/VI in smooth muscle. Concurrent M2-mediated inhibition via Galphai3 and m3-mediated stimulation via Gbetagammaq.
Murthy KS et al (1997) J Biol Chem 272(34) : 21317-24.
Regional binding of 4-diphenylacetoxy-N-methylpiperidine methobromide (4-DAMP) to muscarinic receptors in rat brain and comparative analysis of minimum energy conformations.
Collins D et al (1993) Neurochem Int 22(3) : 237-47.
![4-DAMP [1952-15-4] Chemical Structure](https://cdn.hellobio.com/media/catalog/product//h/b/hb1505.png "4-DAMP [1952-15-4]")
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