Dilution Calculator

The Hello Bio Dilution Calculator gives you an easy way to work out how to dilute stock solutions of known concentrations.

There are 4 parameters: concentration (C1) and volume (V1) of the stock solution and the concentration (C2) and volume (V2) of the desired working solution. Just input any 3 of the 4 parameters – and the calculator will work out the fourth!

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How does it work? The molarity equation

The Hello Bio dilution calculator uses the following equation:

Concentration1 x Volume1 = Concentration2 x Volume2

This can be abbreviated to C1V1 = C2V2

How to use the calculator - a worked example

Below is an example calculation showing what volume of a 10mM stock solution is required to make 50mL of a 100μM working solution.

We will use the equation C1V1=C2V2 where C1=10mM, C2=100μM, V2=50mL and V1 is the unknown

  1. Enter 10 into the 'Concentration 1' box and select millimolar as a unit
  2. Enter 100 into the 'Concentration 2' box and select micromolar as a unit
  3. Enter 50 into the 'Volume 2' box and select milliliter as a unit
  4. Press calculate
  5. The answer of 0.5 milliliter (mL) appears in the 'Volume 1' box

Other useful resources

If you need any further help with handling, storing or solubilising your product, check out our technical FAQs for biochemicals and peptides. These not only give you handy hints on how to use the products safely and store them properly, they also contain all the technical information you need, as well as essential info on solubility and stability.

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